Polypeptides having lysozyme activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9663775-B2
Application number	US-201214360508-A
Country	US
Kind code	B2
Filing date	Nov 23, 2012
Priority date	Nov 25, 2011
Publication date	May 30, 2017
Grant date	May 30, 2017

Abstract

The present invention relates to isolated polypeptides having lysozyme activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

The invention claimed is:

1. A method of isolating DNA from bacteria, comprising:
(a) treating the cell walls of the bacteria with a polypeptide having lysozyme activity, wherein the polypeptide has at least 95% sequence identity to a polypeptide selected from the group consisting of amino acids 20 to 264 of SEQ ID NO: 2, amino acids 21 to 186 of SEQ ID NO: 4, and amino acids 20 to 176 of SEQ ID NO: 6; and
(b) recovering the DNA.

2. A method of producing a polypeptide having lysozyme activity, comprising:
(a) cultivating a recombinant host cell under conditions conducive for production of the polypeptide, wherein
(i) the polypeptide has at least 95% sequence identity to a polypeptide selected from the group consisting of amino acids 20 to 264 of SEQ ID NO: 2, amino acids 21 to 186 of SEQ ID NO: 4, and amino acids 20 to 176 of SEQ ID NO: 6; and
(ii) the recombinant host cell comprises a polynucleotide which encodes the polypeptide and which is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant host cell; and

(b) recovering the polypeptide.

3. The method of claim 2 , wherein the polypeptide has at least 95% sequence identity to amino acids 20 to 264 of SEQ ID NO: 2.

4. The method of claim 2 , wherein the polypeptide has at least 97% sequence identity to amino acids 20 to 264 of SEQ ID NO: 2.

5. The method of claim 2 , wherein the polypeptide has at least 95% sequence identity to amino acids 21 to 186 of SEQ ID NO: 4.

6. The method of claim 2 , wherein the polypeptide has at least 97% sequence identity to amino acids 21 to 186 of SEQ ID NO: 4.

7. The method of claim 2 , wherein the polypeptide has at least 95% sequence identity to amino acids 20 to 176 of SEQ ID NO: 6.

8. The method of claim 2 , wherein the polypeptide has at least 97% sequence identity to amino acids 20 to 176 of SEQ ID NO: 6.

9. The method of claim 2 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5, or the full-length complement of thereof.

10. The method of claim 2 , wherein the polypeptide is a variant of a polypeptide selected from the group consisting of amino acids 20 to 264 of SEQ ID NO: 2, amino acids 21 to 186 of SEQ ID NO: 4, and amino acids 20 to 176 of SEQ ID NO: 6, comprising a substitution, deletion, and/or insertion at one or more positions.

11. The method of claim 2 , wherein the polypeptide is a fragment of a polypeptide selected from the group consisting of amino acids 20 to 264 of SEQ ID NO: 2, amino acids 21 to 186 of SEQ ID NO: 4, and amino acids 20 to 176 of SEQ ID NO: 6, which has lysozyme activity, wherein the fragment has at least 95% sequence identity to a polypeptide selected from the group consisting of amino acids 20 to 264 of SEQ ID NO: 2, amino acids 21 to 186 of SEQ ID NO: 4, and amino acids 20 to 176 of SEQ ID NO: 6.

12. The method of claim 2 , wherein the polypeptide comprises amino acids 20 to 264 of SEQ ID NO: 2.

13. The method of claim 2 , wherein the polypeptide comprises amino acids 21 to 186 of SEQ ID NO: 4.

14. The method of claim 2 , wherein the polypeptide comprises amino acids 20 to 176 of SEQ ID NO: 6.

Assignees

• Novozymes As

Inventors

• Schnorr Kirk Matthew

Classifications

• A61P31/04

  Antibacterial agents · CPC title

• C12Y302/01017

  Lysozyme (3.2.1.17) · CPC title

• A23K50/80

  for aquatic animals, e.g. fish, crustaceans or molluscs · CPC title

• A23K20/189

  Enzymes · CPC title

• C12N15/1003

  Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor · CPC title