Polypeptides having lysozyme activity and compositions comprising it

Patent metadata

Field	Value
Publication number	US-10039300-B2
Application number	US-201715495031-A
Country	US
Kind code	B2
Filing date	Apr 24, 2017
Priority date	Nov 25, 2011
Publication date	Aug 7, 2018
Grant date	Aug 7, 2018

Abstract

The present invention relates to isolated polypeptides having lysozyme activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

The invention claimed is:

1. A granule comprising a polypeptide having lysozyme activity, wherein the granule comprises a core particle comprising the polypeptide and one or more coatings, and wherein the polypeptide has at least 95% sequence identity to a polypeptide sequence having amino acids selected from the group consisting of amino acids 20 to 264 of SEQ ID NO: 2, amino acids 21 to 186 of SEQ ID NO: 4, and amino acids 20 to 176 of SEQ ID NO: 6.

2. The granule of claim 1 , wherein the polypeptide has at least 95% sequence identity to the polypeptide sequence of amino acids 20 to 264 of SEQ ID NO: 2.

3. The granule of claim 1 , wherein the polypeptide has at least 97% sequence identity to the polypeptide sequence of amino acids 20 to 264 of SEQ ID NO: 2.

4. The granule of claim 1 , wherein the polypeptide has at least 95% sequence identity to the polypeptide sequence of amino acids 21 to 186 of SEQ ID NO: 4.

5. The granule of claim 1 , wherein the polypeptide has at least 97% sequence identity to the polypeptide sequence of amino acids 21 to 186 of SEQ ID NO: 4.

6. The granule of claim 1 , wherein the polypeptide has at least 95% sequence identity to the polypeptide sequence of amino acids 20 to 176 of SEQ ID NO: 6.

7. The granule of claim 1 , wherein the polypeptide has at least 97% sequence identity to the polypeptide sequence of amino acids 20 to 176 of SEQ ID NO: 6.

8. The granule of claim 1 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5 or the full-length complement of thereof.

9. The granule of claim 1 , wherein the polypeptide is a variant of a polypeptide sequence having amino acids selected from the group consisting of amino acids 20 to 264 of SEQ ID NO: 2, amino acids 21 to 186 of SEQ ID NO: 4, and amino acids 20 to 176 of SEQ ID NO: 6, comprising a substitution, deletion, and/or insertion at one or more positions.

10. The granule of claim 1 , wherein the polypeptide is a fragment of a polypeptide sequence having amino acids selected from the group consisting of amino acids 20 to 264 of SEQ ID NO: 2, amino acids 21 to 186 of SEQ ID NO: 4, and amino acids 20 to 176 of SEQ ID NO: 6, which has lysozyme activity, wherein the fragment has at least 95% sequence identity to a polypeptide sequence of amino acids selected from the group consisting of amino acids 20 to 264 of SEQ ID NO: 2, amino acids 21 to 186 of SEQ ID NO: 4, and amino acids 20 to 176 of SEQ ID NO: 6.

11. The granule of claim 1 , wherein the polypeptide comprises the sequence of amino acids 20 to 264 of SEQ ID NO: 2.

12. The granule of claim 1 , wherein the polypeptide comprises the sequence of amino acids 21 to 186 of SEQ ID NO: 4.

13. The granule of claim 1 , wherein the polypeptide comprises the sequence of amino acids 20 to 176 of SEQ ID NO: 6.

14. A detergent composition comprising the granule of claim 1 and a surfactant.

15. The detergent composition of claim 14 , which further comprises one or more further enzymes selected from the group comprising of amylases, catalases, cellulases, cutinases, haloperoxygenases, lipases, mannanases, pectinases, pectin lyases, peroxidases, proteases, xanthanases, and xyloglucanases, or any mixture thereof.

16. The detergent composition of claim 14 , further comprising of one or more components selected from the group consisting of builders, hydrotopes, bleaching systems, polymers, fabric hueing agents, adjunct materials, dispersants, dye transfer inhibiting agents, fluorescent whitening agents, soil release polymers and anti-redeposition agents.

17. An animal feed composition comprising the granule of claim 1 .

18. The animal feed composition of claim 17 , which further comprises of one or more amylases; galactanases; alpha-galactosidases; beta-glucanases, phospholipases, phytases, proteases, and xylanases, or any mixture thereof.

19. An animal feed additive comprising
at least one granule of claim 1 ; and
at least one fat-soluble vitamin, and/or
at least one water-soluble vitamin, and/or
at least one trace mineral.

20. The animal feed additive of claim 19 , which further comprises of one or more amylases, galactanases, alpha-galactosidases, beta-glucanases, phospholipases, phytases, proteases, and xylanases, or any mixture thereof.

Assignees

• Novozymes As

Inventors

• Schnorr Kirk Matthew

Classifications

• A61P31/04

  Antibacterial agents · CPC title

• A23K40/25

  by extrusion · CPC title

• C12Y302/01017

  Lysozyme (3.2.1.17) · CPC title

• A23K20/189 Primary

  Enzymes · CPC title

• C12N15/1003

  Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor · CPC title