Mass spectrometry analysis of microorganisms in samples

Patent metadata

Field	Value
Publication number	US-10811241-B2
Application number	US-202016809171-A
Country	US
Kind code	B2
Filing date	Mar 4, 2020
Priority date	Jun 25, 2013
Publication date	Oct 20, 2020
Grant date	Oct 20, 2020

Abstract

The invention generally relates to methods for analyzing an analyte in a sample. In certain embodiments, the methods involve providing a capture module, the module configured to capture an analyte in a sample in an ambient environment and generate ions of the analyte, wherein the capture module comprises a cartridge and a porous substrate within the cartridge that is connected to a voltage source, and wherein the porous substrate further comprises an internal standard; capturing the analyte in the sample to the porous substrate of the capture module; generating ions of the analyte and the internal standard held by the porous substrate via the voltage source that is coupled to the porous substrate; and analyzing the generated ions of the analyte and the internal standard in a mass analyzer that is operably coupled to the capture module.

First claim

What is claimed is:

1. A method for analyzing an analyte in a sample, the method comprising:
providing a capture module, the module configured to capture an analyte in a sample in an ambient environment and generate ions of the analyte, wherein the capture module comprises a cartridge and a porous substrate within the cartridge that is connected to a voltage source, and wherein the porous substrate further comprises an internal standard;
capturing the analyte in the sample to the porous substrate of the capture module;
generating ions of the analyte and the internal standard held by the porous substrate via the voltage source that is coupled to the porous substrate; and
analyzing the generated ions of the analyte and the internal standard in a mass analyzer that is operably coupled to the capture module.

2. The method according to claim 1 , wherein the method further comprises directing a gas-flow through the porous substrate.

3. The method according to claim 1 , further comprising applying a solvent to the porous substrate.

4. The method according to claim 1 , wherein the porous substrate is filter paper.

5. The method according to claim 4 , wherein the filter paper tapers to a distal tip.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Hamid Ahmed Mohamed
• Jarmusch Alan Keith
• Ouyang Zheng

Classifications

• C12N13/00 Primary

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• C12Q1/04

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• H01J49/0445 Primary

  with means for introducing as a spray, a jet or an aerosol (electrospray ion sources H01J49/165) · CPC title

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title

• H01J49/16

  using surface ionisation, e.g. field-, thermionic- or photo-emission · CPC title