Means and methods for yield performance in plants

Patent metadata

Field	Value
Publication number	US-10801032-B2
Application number	US-201414895918-A
Country	US
Kind code	B2
Filing date	Jun 3, 2014
Priority date	Jun 3, 2013
Publication date	Oct 13, 2020
Grant date	Oct 13, 2020

Abstract

This disclosure relates to the field of plant molecular biology; more particularly to the field of agriculture; even more particularly to the field of improving the yield of plants. This disclosure provides chimeric genes and constructs that can be used to enhance the yield in plants and crops.

First claim

The invention claimed is:

1. A method for producing a plant cell, wherein the method comprises:
identifying a promoter region of a plant GA2 oxidase gene that is active in the growth zone of a plant leaf,
identifying a DNA region encoding a plant CYP78A5 protein;
operatively linking the identified promoter to the identified DNA region encoding a plant CYP78A5 protein and a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of a plant to form a chimeric gene;
introducing into or transforming a plant cell with the chimeric gene,
selecting a plant cell with a stable expression of the chimeric gene . and
determining that a plant generated from the selected plant cell has an increased yield as compared to a corresponding wildtype plant.

2. The method according to claim 1 , wherein the plant cell is a plant cell of a crop plant.

3. The method according to claim 2 , wherein the plant cell is a plant cell of a cereal plant.

4. The method according to claim 2 , wherein the plant cell is a plant cell of a grass.

5. The method according to claim 1 , further comprising, generating a seed or a plant tissue comprising the plant cell.

Assignees

• Vib Vzw
• Univ Gent

Inventors

• Inzé Dirk Gustaaf
• Claeys Hannes
• Nelissen Hilde
• Sun Xiaohuan

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8229 Primary

  Meristem-specific, e.g. nodal, apical · CPC title

• C12N15/8261 Primary

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• C12Y114/11013

  Gibberellin 2-beta-dioxygenase (1.14.11.13) · CPC title