Compositions and methods for altering flowering and plant architecture to improve yield potential

Patent metadata

Field	Value
Publication number	US-10294486-B2
Application number	US-201615131987-A
Country	US
Kind code	B2
Filing date	Apr 18, 2016
Priority date	Apr 20, 2015
Publication date	May 21, 2019
Grant date	May 21, 2019

Abstract

The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.

First claim

What is claimed is:

1. A recombinant DNA construct comprising a polynucleotide sequence encoding a florigenic FT protein comprising an amino acid sequence having at least 80% identity to SEQ ID NO: 2 and operably linked to a promoter comprising a polynucleotide sequence having at least 90% identity to SEQ ID NO: 21, SEQ ID NO: 22 or SEQ ID NO: 38.

2. The recombinant DNA construct of claim 1 , wherein said florigenic FT protein comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 2.

3. The recombinant DNA construct of claim 1 , wherein said florigenic FT protein comprises an amino acid sequence having at least 95% identity to SEQ ID NO: 2.

4. The recombinant DNA construct of claim 1 , wherein said florigenic FT protein comprises the amino acid sequence SEQ ID NO: 2.

5. The recombinant DNA construct of claim 1 , wherein said promoter comprises a polynucleotide sequence having at least 90% identity to SEQ ID NO: 21.

6. The recombinant DNA construct of claim 1 , wherein said promoter comprises SEQ ID NO: 21.

7. The recombinant DNA construct of claim 1 , wherein said promoter comprises a polynucleotide sequence having at least 90% identity to SEQ ID NO: 22 or SEQ ID NO: 38.

8. The recombinant DNA construct of claim 1 , wherein said promoter comprises SEQ ID NO: 22 or SEQ ID NO: 38.

9. The recombinant DNA construct of claim 1 , wherein said florigenic FT protein is encoded by a polynucleotide comprising at least 90% identity to SEQ ID NO:1.

10. The recombinant DNA construct of claim 1 , wherein said recombinant DNA construct comprises SEQ ID NO:1.

11. A DNA molecule or vector comprising the recombinant DNA construct of claim 1 .

12. A transgenic plant, or part thereof, wherein the plant or plant part thereof comprises the recombinant DNA construct of claim 1 .

13. The transgenic plant, or part thereof, of claim 12 , wherein the transgenic plant or part thereof is homozygous for the insertion of the recombinant DNA construct.

14. The transgenic plant, or part thereof, of claim 12 , wherein the transgenic plant or part thereof is hemizygous for the insertion of the recombinant DNA construct.

15. The transgenic plant, or part thereof, of claim 12 , wherein the transgenic plant or a plant grown from the part thereof is a short day plant.

16. The transgenic plant, or part thereof, of claim 12 , wherein the transgenic plant or a plant grown from the part thereof is a dicotyledonous plant.

17. The transgenic plant, or part thereof, of claim 16 , wherein the transgenic plant or a plant grown from the part thereof is a leguminous plant.

18. The transgenic plant, or part thereof, of claim 17 , wherein the transgenic plant or a plant grown from the part thereof is soybean.

19. The transgenic plant, or part thereof, of claim 18 , wherein the transgenic soybean plant or transgenic soybean plant grown from the part thereof produces more pods per node than a control plant not having the recombinant DNA construct.

20. The transgenic plant, or part thereof, of claim 12 , wherein the transgenic plant or a plant grown from the part thereof produces more flowers per node than a control plant not having the recombinant DNA construct.

21. The transgenic plant, or part thereof, of claim 12 , wherein the transgenic plant or a plant grown from the part thereof produces more bolls, siliques, fruits, nuts or pods per node of the transgenic plant than a control plant not having the recombinant DNA construct.

22. The transgenic plant, or part thereof, of claim 12 , wherein the transgenic plant or a plant grown from the part thereof flowers earlier than a control plant not having the recombinant DNA construct.

23. The transgenic plant or part thereof of claim 12 , wherein the transgenic plant or a plant grown from the part thereof has more floral racemes per node than a control plant not having the recombinant DNA construct.

24. The transgenic plant part of claim 12 , wherein the transgenic plant part comprises the recombinant DNA construct and is one of the following: a seed, fruit, leaf, cotyledon, hypocotyl, meristem, embryo, endosperm, root, shoot, stem, pod, flower, inflorescence, stalk, pedicel, style, stigma, receptacle, petal, sepal, pollen, anther, filament, ovary, ovule, pericarp, phloem, or vascular tissue.

Assignees

• Monsanto Technology Llc

Inventors

• Brower-Toland Brent
• Caldo Rico A
• Dai Shunhong
• Gabbert Karen
• Goldshmidt Alexander
• Howell Miya D
• Karunanandaa Balasulojini
• Manjunath Sivalinganna
• Mcdill Bradley W
• Ovadya Daniel J
• Preuss Sasha
• Rice Elena A
• Savidge Beth
• Sharma Vijay K

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/827 Primary

  Flower development or morphology, e.g. flowering promoting factor [FPF] · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8229

  Meristem-specific, e.g. nodal, apical · CPC title