Plant promoter for transgene expression

Patent metadata

Field	Value
Publication number	US-10280429-B2
Application number	US-201615298467-A
Country	US
Kind code	B2
Filing date	Oct 20, 2016
Priority date	Oct 22, 2015
Publication date	May 7, 2019
Grant date	May 7, 2019

Abstract

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Zea mays KN1 gene. Some embodiments relate to a promoter from a Zea mays KN1 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

First claim

What is claimed is:

1. A nucleic acid vector comprising a promoter operably linked to a heterologous polynucleotide sequence of:
a) a polylinker sequence;
b) a non- Zea mays KNI gene; or
c) a combination of a) and b), wherein said promoter comprises a polynucleotide sequence that has at least 99% sequence identity with SEQ ID NO:1.

2. The nucleic acid vector of claim 1 , wherein said promoter is 1,407 bp in length.

3. The nucleic acid vector of claim 1 , wherein said promoter consists of a polynucleotide sequence that has at least 99% sequence identity with SEQ ID NO:1.

4. The nucleic acid vector of any one of claims 1 - 3 , further comprising a sequence encoding a selectable marker.

5. The nucleic acid vector of claim 1 , wherein said promoter is operably linked to a transgene.

6. The nucleic acid vector of claim 5 , wherein the transgene encodes a selectable marker or a gene product conferring insecticidal resistance, herbicide tolerance, nitrogen use efficiency, water use efficiency, expression of an RNAi, or nutritional quality.

7. The nucleic acid vector of any of claim 1 - 3 , or 5 , further comprising a 3′ untranslated polynucleotide sequence.

8. The nucleic acid vector of any of claim 1 - 3 , or 5 , further comprising an operably linked 5′ untranslated polynucleotide sequence.

9. The nucleic acid vector of any of claim 1 - 3 , or 5 , further comprising an intron sequence.

10. The nucleic acid vector of claim 1 , wherein said promoter has reproductive meristematic tissue specific expression.

11. A transgenic plant comprising a polynucleotide sequence that has at least 99% sequence identity with SEQ ID NO:1 operably linked to a transgene.

12. The transgenic plant of claim 11 , wherein said plant is selected from the group consisting of Zea mays , wheat, rice, sorghum, oats, rye, bananas, sugar cane, soybean, cotton, Arabidopsis , tobacco, sunflower, and canola.

13. The transgenic plant of claim 12 , wherein said plant is Zea mays.

14. The transgenic plant of claim 11 , further comprising a 3′ untranslated sequence.

15. The transgenic plant of claim 11 , wherein said transgene is expressed specifically in reproductive meristematic.

16. The transgenic plant of claim 11 , wherein said polynucleotide sequence is 1,407 bp in length.

17. A method for producing a transgenic plant cell, the method comprising the steps of:
a) transforming a plant cell with a gene expression cassette comprising a Zea mays KN1 promoter, wherein said Zea mays KN1 promoter comprises a polynucleotide sequence that has at least 99% sequence identity with SEQ ID NO:1, operably linked to at least one polynucleotide sequence of interest;
b) isolating the transformed plant cell comprising the gene expression cassette; and,
c) producing a transgenic plant cell comprising the Zea mays KN1 promoter operably linked to at least one polynucleotide sequence of interest.

18. The method of claim 17 , wherein transforming a plant cell is performed with a plant transformation method.

19. The method of claim 18 , wherein the plant transformation method is selected from the group consisting of an Agrobacterium -mediated transformation method, a biolistics transformation method, a silicon carbide transformation method, a protoplast transformation method, and a liposome transformation method.

20. The method of claim 17 , wherein the polynucleotide sequence is expressed specifically in reproductive meristematic.

21. The method of claim 17 , wherein the Zea mays KN1 promoter operably linked to at least one polynucleotide sequence of interest is stably integrated into the genome of the transgenic plant cell.

22. The method of claim 17 , the method further comprising the steps of:
d) regenerating the transgenic plant cell into a transgenic plant; and,
e) obtaining the transgenic plant, wherein the transgenic plant comprises the gene expression cassette comprising the Zea mays KN1 promoter operably linked to at least one polynucleotide sequence of interest.

23. The method of claim 17 , wherein the transgenic plant cell is a monocotyledonous transgenic plant cell or a dicotyledonous transgenic plant cell.

24. The method of claim 23 , wherein the dicotyledonous transgenic plant cell is selected from the group consisting of an Arabidopsis plant cell, a tobacco plant cell, a soybean plant cell, a canola plant cell, and a cotton plant cell.

25. The method of claim 23 , wherein the monocotyledonous transgenic plant cell is selected from the group consisting of a Zea mays plant cell, a rice plant cell, and a wheat plant cell.

26. The Zea mays KN1 promoter of claim 17 , the Zea mays KN1 promoter comprising the polynucleotide of SEQ ID NO:1.

Assignees

• Dow Agrosciences Llc

Inventors

• Davies John
• Mann David
• Connell James Patrick
• Beeson Iv William T

Classifications

• C12N15/8233

  Female-specific, e.g. pistil, ovule · CPC title

• C12N15/8229 Primary

  Meristem-specific, e.g. nodal, apical · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/8241

  Phenotypically and genetically modified plants via recombinant DNA technology · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title