Enclosed desorption electrospray ionization probes and method of use thereof

Patent metadata

Field	Value
Publication number	US-10799165-B2
Application number	US-201916720991-A
Country	US
Kind code	B2
Filing date	Dec 19, 2019
Priority date	Jun 3, 2011
Publication date	Oct 13, 2020
Grant date	Oct 13, 2020

Abstract

The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

First claim

What is claimed is:

1. An ionization device, the device comprising:
a pump;
a U-shaped elongate tube comprising a proximal end connected to the pump and an open distal end, wherein the elongate tube further comprises a first opening in a sidewall located at a distal portion of the elongate tube and a second opening in the sidewall configured to mate with an inlet of a mass spectrometer; and
a desorption electrospray ionization (DESI) source comprising a discharge tube;
wherein the device is configured such that a portion of the discharge tube is inserted through the first opening in the sidewall of the elongate tube and terminates prior to the open distal end.

2. The ionization device of claim 1 , wherein the DESI source produces a DESI-active spray in a first direction within the elongate tube, and a velocity of the DESI-active spray in the first direction is reduced by a gas flow in an opposite direction generated in the elongate tube by the pump.

3. The ionization device of claim 1 , wherein the elongate tube is a multi-segmented tube.

4. The ionization device of claim 1 , wherein the portion of the discharge tube is sealed within the elongate tube.

5. The ionization device of claim 1 , wherein the distal portion of the elongate tube is configured to interact with a surface.

6. The ionization device of claim 5 , wherein the surface is tissue.

7. The ionization device of claim 2 , wherein the mass spectrometer is a bench top mass spectrometer or a handheld mass spectrometer.

8. The ionization device of claim 2 , wherein the DESI-active spray is generated by an electrospray device.

9. The ionization device of claim 2 , wherein the source produces the DESI-active spray without voltage.

10. The ionization device of claim 2 , wherein the DESI-active spray comprises water.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Ouyang Zheng
• Chen Chien-Hsun
• Lin Ziqing
• Eberlin Livia Schiavinato

Classifications

• A61B10/0045 Primary

  Devices for taking samples of body liquids · CPC title

• G01N2001/028

  Sampling from a surface, swabbing, vaporising · CPC title

• A61B5/157

  {Devices} characterised by integrated means for measuring characteristics of blood · CPC title

• A61B5/150992

  Blood sampling from a fluid line external to a patient, such as a catheter line, combined with an infusion line; Blood sampling from indwelling needle sets, e.g. sealable ports, luer couplings or valves · CPC title

• G01N33/4833

  of solid biological material, e.g. tissue samples, cell cultures (tissue in vivo A61B5/00; cell suspensions G01N33/48735) · CPC title