Enclosed desorption electrospray ionization probes and method of use thereof

Patent metadata

Field	Value
Publication number	US-10004440-B2
Application number	US-201715617013-A
Country	US
Kind code	B2
Filing date	Jun 8, 2017
Priority date	Jun 3, 2011
Publication date	Jun 26, 2018
Grant date	Jun 26, 2018

Abstract

The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

First claim

What is claimed is:

1. A method for analyzing a tissue section, the method comprising:
providing a tissue section;
conducting a mass spectrometry technique on the tissue section that comprises directing a liquid droplet spray discharge from a sampling probe onto the tissue section, wherein the liquid droplet spray discharge from the sampling probe desorbs and ionizes an analyte from the tissue section, and wherein the mass spectrometry technique is conducted in a manner that preserves the tissue section further analysis;
transferring the ions of the analyte to a mass spectrometer; and
analyzing the ions of the analyte in the mass spectrometer, thereby analyzing the tissue section.

2. The method according to claim 1 , wherein the method is repeated at least a second time on the same tissue section.

3. The method according to claim 1 , wherein the discharge is water.

4. The method according to claim 1 , wherein the sampling probe operates without voltage.

5. The method according to claim 1 , wherein the transferring step comprises sending the ions of the analyte through a transfer member that is operably coupled to the mass spectrometer.

6. The method according to claim 5 , wherein the method further comprises generating a gas flow in the transfer member via a pump in a direction opposite that of a first direction with enough counter-flow to reduce a velocity of the discharge.

7. The method accordingly to claim 6 , wherein a distal portion of the sampling probe is sealed within the transfer member.

8. The method according to claim 7 , wherein the distal portion of the transfer member is configured to sealably interact with the tissue section.

9. The method according to claim 1 , wherein the mass spectrometer is a bench top mass spectrometer or a handheld mass spectrometer.

10. The method according to claim 1 , wherein the tissue section is a brain tissue section.

11. A method for analyzing a tissue section, the method comprising:
providing a tissue section;
conducting a mass spectrometry technique on the tissue section that comprises directing a liquid droplet spray discharge from a sampling probe onto the tissue section, wherein the liquid droplet spray discharge from the sampling probe desorbs and ionizes a lipid from the tissue section, and wherein the mass spectrometry technique is conducted in a manner that preserves the tissue section for further analysis;
transferring the ions of the lipid to a mass spectrometer; and
analyzing the ions of the lipid in the mass spectrometer, thereby analyzing the tissue section.

12. The method according to claim 11 , wherein the method is repeated at least a second time on the same thin tissue section.

13. The method according to claim 11 , wherein the discharge is water.

14. The method according to claim 11 , wherein the sampling probe operates without voltage.

15. The method according to claim 11 , wherein the transferring step comprises sending the ions of the analyte through a transfer member that is operably coupled to the mass spectrometer.

16. The method according to claim 15 , wherein the method further comprises generating a gas flow in the transfer member via a pump in a direction opposite that of a first direction with enough counter-flow to reduce a velocity of the discharge.

17. The method accordingly to claim 16 , wherein a distal portion of the sampling probe is sealed within the transfer member.

18. The method according to claim 17 , wherein the distal portion of the transfer member is configured to sealably interact with the tissue section.

19. The method according to claim 11 , wherein the mass spectrometer is a bench top mass spectrometer or a handheld mass spectrometer.

20. The method according to claim 11 , wherein the tissue section is a brain tissue section.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Ouyang Zheng
• Chen Chien-Hsun
• Lin Ziqing
• Eberlin Livia Schiavinato

Classifications

• H01J49/167

  Capillaries and nozzles specially adapted therefor; (electrostatic spraying per se B05B5/00) · CPC title

• A61M2202/0468

  non-physiological · CPC title

• H01J49/0404

  Capillaries used for transferring samples or ions (electrospray nozzles H01J49/167) · CPC title

• A61B5/157

  {Devices} characterised by integrated means for measuring characteristics of blood · CPC title

• A61B5/145

  Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value {; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue} (non-radiation detecting or locating of foreign bodies in blood A61B5/06) · CPC title