Desorption electrospray ionization sampling without damaging an in vivo tissue sample

Patent metadata

Field	Value
Publication number	US-9538945-B2
Application number	US-201514688496-A
Country	US
Kind code	B2
Filing date	Apr 16, 2015
Priority date	Jun 3, 2011
Publication date	Jan 10, 2017
Grant date	Jan 10, 2017

Abstract

The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

First claim

What is claimed is:

1. A method for analyzing in vivo tissue, the method comprising:
generating from a sampling probe, a liquid droplet spray discharge that is non-toxic to in vivo tissue;
directing the liquid droplet spray discharge onto the in vivo tissue, wherein the liquid droplet spray discharge from the sampling probe desorbs and ionizes an analyte from the in vivo tissue in a manner that does not damage the in vivo tissue;
transferring the ions of the analyte to a mass spectrometer; and
analyzing the ions of the analyte in the mass spectrometer, thereby analyzing the in vivo tissue.

2. The method according to claim 1 , wherein the discharge is water.

3. The method according to claim 1 , wherein the sampling probe operates without voltage.

4. The method according to claim 1 , wherein the transferring step comprises sending the ions of the analyte through a transfer member that is operably coupled to the mass spectrometer.

5. The method according to claim 4 , wherein the method further comprises generating a gas flow in the transfer member via a pump in a direction opposite that of a first direction with enough counter-flow to reduce a velocity of the discharge.

6. The method according to claim 4 , wherein the transfer member is a catheter.

7. The method accordingly to claim 6 , wherein a distal portion of the sampling probe is sealed within the transfer member.

8. The method according to claim 7 , wherein the distal portion of the transfer member is configured to sealably interact with the in vivo tissue.

9. The method according to claim 1 , wherein the mass spectrometer is a bench top mass spectrometer or a handheld mass spectrometer.

10. The method according to claim 1 , wherein the in vivo tissue is human in in vivo tissue.

11. A system for analyzing an in vivo tissue, the system comprising:
a discharge source that operates without voltage and that produces a liquid droplet spray discharge that desorbs and ionizes an analyte from a sample in a manner that does not damage the in vivo tissue;
a transfer member;
a mass spectrometer; and
a pump;
wherein the system is configured such that the transfer member is coupled to the mass spectrometer and the pump, a distal portion of the source is enclosed within the transfer member such that the liquid droplet spray discharge is produced within the transfer member in a first direction and its velocity is reduced by the pump, which produces a gas flow in the transfer member in a direction opposite that of the first direction, and a gas flow produced by the system transfers ions through the transfer member to the mass spectrometer.

12. The system according to claim 11 , wherein the transfer member is a catheter.

13. The system according to claim 11 , wherein the distal portion is sealed within the transfer member.

14. The system according to claim 11 , wherein the distal portion of the transfer member is configured to sealably interact with the in vivo tissue.

15. The system according to claim 11 , wherein the mass spectrometer is a bench top mass spectrometer or a handheld mass spectrometer.

16. The system according to claim 11 , wherein the mass spectrometer comprises a mass analyzer that is selected from the group consisting of: a quadrupole ion trap, a rectalinear ion trap, a cylindrical ion trap, a ion cyclotron resonance trap, an ORBITRAP mass spectrometer (ion trap mass analyzer consisting of an outer barrel-like electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the spindle), a time of flight, a Fourier Transform ion cyclotron resonance, and sectors.

17. The system according to claim 11 , wherein the discharge source comprises a source that produces a DESI-active spray.

18. The system according to claim 11 , wherein the source of DESI-active spray is an electrospray device.

19. The system according to claim 11 , wherein the discharge is water.

20. The system according to claim 11 , wherein a porous protective member covers a distal end of the source.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Ouyang Zheng
• Chen Chien-Hsun
• Lin Ziqing
• Eberlin Livia Schiavinato

Classifications

• A61B5/157

  {Devices} characterised by integrated means for measuring characteristics of blood · CPC title

• A61B5/145

  Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value {; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue} (non-radiation detecting or locating of foreign bodies in blood A61B5/06) · CPC title

• A61M2202/0468

  non-physiological · CPC title

• A61B5/150015

  Source of blood · CPC title

• H01J49/0404

  Capillaries used for transferring samples or ions (electrospray nozzles H01J49/167) · CPC title