Method for treating pyropheophytin-containing compositions
US-9493748-B2 · Nov 15, 2016 · US
Polypeptides having phospholipase C activity and polynucleotides encoding same
US10745676B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10745676-B2 |
| Application number | US-201816152043-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 4, 2018 |
| Priority date | Dec 11, 2012 |
| Publication date | Aug 18, 2020 |
| Grant date | Aug 18, 2020 |
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- Title
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Abstract
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The present invention relates to isolated polypeptides having phospholipase C activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
First claim
Opening claim text (preview).
The invention claimed is: 1. A nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having phospholipase C activity, wherein the polypeptide has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2 and the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in an expression host. 2. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2. 3. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2. 4. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide comprises the sequence of amino acid residues 1-594 of SEQ ID NO: 2. 5. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide is a fragment of the sequence of amino acid residues 1-594 of SEQ ID NO: 2. 6. A recombinant host cell comprising the nucleic acid construct or expression vector of claim 1 . 7. A recombinant host cell comprising the nucleic acid construct or expression vector of claim 2 . 8. A recombinant host cell comprising the nucleic acid construct or expression vector of claim 3 . 9. A recombinant host cell comprising the nucleic acid construct or expression vector of claim 4 . 10. A recombinant host cell comprising the nucleic acid construct or expression vector of claim 5 . 11. A method of producing a polypeptide having phospholipase C activity, comprising: (a) cultivating the recombinant host cell of claim 6 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide. 12. A method of producing a polypeptide having phospholipase C activity, comprising: (a) cultivating the recombinant host cell of claim 7 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide. 13. A method of producing a polypeptide having phospholipase C activity, comprising: (a) cultivating the recombinant host cell of claim 8 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide. 14. A method of producing a polypeptide having phospholipase C activity, comprising: (a) cultivating the recombinant host cell of claim 9 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide. 15. A method of producing a polypeptide having phospholipase C activity, comprising: (a) cultivating the recombinant host cell of claim 10 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
Assignees
Inventors
Classifications
- C11B3/003Primary
by enzymes or microorganisms, living or dead · CPC title
with acids · CPC title
Other fatty acid esters, e.g. phosphatides · CPC title
involving modified lipid metabolism, e.g. seed oil composition · CPC title
Phospholipase C (3.1.4.3) · CPC title
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Frequently asked questions
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- What does patent US10745676B2 cover?
- The present invention relates to isolated polypeptides having phospholipase C activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
- Who is the assignee on this patent?
- Novozymes As
- What technology area does this patent fall under?
- Primary CPC classification C11B3/003. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 18 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).