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Method for treating pyropheophytin-containing compositions
US9493748B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9493748-B2 |
| Application number | US-201013321869-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 10, 2010 |
| Priority date | Jun 12, 2009 |
| Publication date | Nov 15, 2016 |
| Grant date | Nov 15, 2016 |
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Abstract
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The present invention provides methods and uses for treating pyropheophytin-containing compositions, particularly in order to remove pyropheophytin therefrom. The compositions are typically plant, algal, or bacterial derived products such as vegetable oils. The method comprises a step of contacting the compositions with an enzyme having pyropheophytinase activity. Also provided are related apparatus and products for performing such methods and uses.
First claim
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The invention claimed is: 1. A method for treating a pyropheophytin-containing plant oil, comprising contacting the oil with an enzyme which hydrolyses pyropheophytin, wherein the enzyme has a pheophytinase to pyropheophytinase activity ratio of less than 50, wherein the enzyme comprises an amino acid sequence having at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 21, 23 or 25 wherein the oil is heterologous to the enzyme and wherein as a result of the enzyme's contact with the oil, the concentration of pyropheophytin in the treated oil is reduced by at least 10% compared to the concentration of pyropheophytin present in the oil before treatment. 2. The method according to claim 1 , wherein the enzyme has pheophytinase or pheophytin pheophorbide hydrolase activity. 3. The method according to claim 1 , wherein the enzyme is from Chlamydomonas reinhardtii or Triticum aestivum. 4. The method according to claim 1 , wherein pyropheophytin in the oil is hydrolysed to form pyropheophorbide, and the method further comprises a step of removing pyropheophorbide from the oil. 5. The method according to claim 4 , wherein the method comprises a deodorization step. 6. The method according to claim 4 , wherein the method comprises a step of silica treatment. 7. The method according to claim 6 , wherein the method comprises two or more silica treatment steps. 8. The method according to claim 6 , wherein the silica treatment is performed at 70 to 110° C. 9. The method according to claim 1 , wherein the oil is selected from rice bran, soy, canola, palm, olive, cottonseed, corn, palm kernel, coconut, peanut, sesame or sunflower oil. 10. The method according to claim 1 , further comprising a hexane extraction and/or degumming step.
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- What does patent US9493748B2 cover?
- The present invention provides methods and uses for treating pyropheophytin-containing compositions, particularly in order to remove pyropheophytin therefrom. The compositions are typically plant, algal, or bacterial derived products such as vegetable oils. The method comprises a step of contacting the compositions with an enzyme having pyropheophytinase activity. Also provided are related appa…
- Who is the assignee on this patent?
- Søe Jørn Borch, Poulsen Charlotte Horsmans, Zargahi Masoud Rajabi, and 6 more
- What technology area does this patent fall under?
- Primary CPC classification C12N9/18. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 15 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).