High-activity mutants of butyrylcholinesterase for cocaine hydrolysis and method of generating the same
US-9206403-B1 · Dec 8, 2015 · US
Process modifications to enhance chlorophyll degradation
US9493749B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9493749-B2 |
| Application number | US-201214000970-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 16, 2012 |
| Priority date | Feb 23, 2011 |
| Publication date | Nov 15, 2016 |
| Grant date | Nov 15, 2016 |
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Abstract
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In one aspect, there is provided a process for treating a plant oil, comprising a step of contacting the oil with an enzyme, wherein the enzyme is capable of hydrolyzing an a′ or b′ stereoisomer of chlorophyll or a chlorophyll derivative.
First claim
Opening claim text (preview).
The invention claimed is: 1. A process for treating a plant oil comprising a step of contacting the oil with an enzyme, wherein the enzyme is capable of hydrolysing an a′ or b′ stereoisomer of chlorophyll or a chlorophyll derivative and wherein the enzyme comprises a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 13. 2. The process according to claim 1 , wherein the a′ or b′ stereoisomer is chlorophyll a′, pheophytin a′, chlorophyll b′ or pheophytin b′. 3. The process according to claim 1 , wherein the enzyme is capable of hydrolysing an a′ stereoisomer of chlorophyll or the chlorophyll derivative. 4. The process according to claim 1 , wherein the enzyme has an activity ratio of less than 10, where said activity ratio is (a) the ratio of the activity of the enzyme on the a stereoisomer of chlorophyll or the chlorophyll derivative to the activity of the enzyme on the a′ stereoisomer of chlorophyll or chlorophyll derivative, or (b) the ratio of the activity of the enzyme on the b stereoisomer of chlorophyll or chlorophyll derivative to the activity of the enzyme on the b′ stereoisomer of chlorophyll or the chlorophyll derivative. 5. The process according to claim 1 , wherein following treatment with the enzyme the oil comprises (a) at least 50% a stereoisomers of chlorophyll or the chlorophyll derivative, based on the total amount of a and a′ stereoisomers of chlorophyll or the chlorophyll derivative in the oil; or (b) at least 50% b stereoisomers of chlorophyll or the chlorophyll derivative, based on the total amount of b and b′ stereoisomers of chlorophyll or the chlorophyll derivative in the oil. 6. The process according to claim 1 , wherein the enzyme has an activity ratio of less than 10, wherein said activity ratio is the ratio of the activity of the enzyme on pheophytin to the activity of the enzyme on pyropheophytin. 7. The process according to claim 1 , wherein the enzyme comprises a chlorophyllase, pheophytinase and/or pyropheophytinase activity. 8. The process according to claim 1 , wherein the enzyme comprises the amino acid sequence GHSRG (SEQ ID NO: 32). 9. The process according to claim 1 , wherein the enzyme is derived from Ricinus communis.
Assignees
Inventors
Classifications
using catalysts, e.g. selective catalysts · CPC title
Glycerides · CPC title
by enzymes or microorganisms, living or dead · CPC title
Chlorophyllase (3.1.1.14) · CPC title
- C12N9/18Primary
Carboxylic ester hydrolases {(3.1.1)} · CPC title
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Frequently asked questions
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- What does patent US9493749B2 cover?
- In one aspect, there is provided a process for treating a plant oil, comprising a step of contacting the oil with an enzyme, wherein the enzyme is capable of hydrolyzing an a′ or b′ stereoisomer of chlorophyll or a chlorophyll derivative.
- Who is the assignee on this patent?
- Soe Jorn Borch, Jorgensen Tina Lillan, Lauridsen Lene, and 3 more
- What technology area does this patent fall under?
- Primary CPC classification C12N9/18. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 15 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).