Method and apparatus for sorting particles

Patent metadata

Field	Value
Publication number	US-10710120-B2
Application number	US-201816014895-A
Country	US
Kind code	B2
Filing date	Jun 21, 2018
Priority date	Apr 17, 2002
Publication date	Jul 14, 2020
Grant date	Jul 14, 2020

Abstract

A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises actuators and chambers for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.

First claim

Having described the invention, what is claimed as new and protected by Letters Patent is:

1. A particle sorting system, comprising:
a duct configured to convey particles in a stream comprising an inlet, a first outlet and a second outlet;
a sensor for sensing a predetermined characteristic of a particle flowing in the duct;
a first chamber having a movable wall fluidically couplable to the duct;
a second chamber having a movable wall fluidically couplable to the duct, the second chamber opposing the first chamber on an opposite side of the duct; and
an actuator operatively coupled to at least one of the first chamber or the second chamber,
wherein movement of the movable wall in the first chamber or the second chamber causes the first chamber or the second chamber to transiently discharge an amount of fluid into the duct such that the transient discharge of fluid pressure does not halt or disrupt flow volume through the first outlet or the second outlet.

2. The particle sorting system of claim 1 wherein the actuator operates to compress fluid in at least one of the first chamber or the second chamber to deflect one of the particles in the stream into one of the first outlet or the second outlet.

3. The particle sorting system of claim 2 , wherein the actuator operates to compress fluid upon sensing of the predetermined characteristic by the sensor.

4. The particle sorting system of claim 1 , further comprising a first side channel connecting the duct with the first chamber.

5. The particle sorting system of claim 4 , further comprising a second side channel connecting the duct with the second chamber.

6. The particle sorting system of claim 5 , wherein the first side channel and the second side channel are positioned upstream of the first outlet and the second outlet.

7. The particle sorting system of claim 1 , wherein operation of the actuator to compress fluid in at least one of the first chamber or the second chamber causes flow through the duct to be transiently moved sideways back and forth upon pressurizing and depressurizing of the first chamber or second chamber.

8. The particle sorting system of claim 1 , wherein the actuator is a piezoelectric actuator bonded to the movable wall of the first chamber or the movable wall of the second chamber.

9. The particle sorting system of claim 1 , wherein the first chamber and the second chamber are filled with fluid.

10. The particle sorting system of claim 7 , wherein the transient flow includes a component perpendicular to a normal flow of the particle through the duct.

11. The particle sorting system of claim 1 , wherein the first chamber or the second chamber acts as a buffer for absorbing pressure transients.

12. The particle sorting system of claim 1 , wherein the first chamber and the second chamber hydraulically co-operate with each other.

13. The particle sorting system of claim 1 , wherein the movable wall of the first chamber and the second chamber have resilient properties.

14. The particle sorting system of claim 1 , wherein the sensor includes an optical sensor.

15. The particle sorting system of claim 1 , wherein the predetermined characteristic of the particle flowing in the duct is velocity.

16. The particle sorting system of claim 1 , wherein the particles flow in the stream in a sheath fluid.

17. The particle sorting system of claim 1 , wherein the actuator is an external actuator.

18. The particle sorting system of claim 1 , wherein the first chamber and the second chamber are configured such that the pressure inside the first chamber is equalized to the pressure in the second chamber subsequent to the transient discharge of fluid pressure.

19. The particle sorting system of claim 1 , wherein the transient discharge of fluid pressure shifts a location of the particle flowing in the duct perpendicular to a flow direction of the particle.

20. The particle sorting system of claim 1 , wherein an angle between a first branch leading to the first outlet and a second branch leading to the second outlet is in a range between 10 and 45 degrees.

Assignees

• Cytonome St Llc

Inventors

• Deshpande Manish
• Gilbert John R

Classifications

• B07C5/34 Primary

  Sorting according to other particular properties {(material testing per se G01N; quality control G07C3/14)} · CPC title

• F16K99/0001

  Microvalves (microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006) · CPC title

• G01N15/14

  Optical investigation techniques, e.g. flow cytometry · CPC title

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B01L2300/0877

  Flow chambers · CPC title