Method and apparatus for sorting particles

Patent metadata

Field	Value
Publication number	US-9550215-B2
Application number	US-201414281303-A
Country	US
Kind code	B2
Filing date	May 19, 2014
Priority date	Apr 17, 2002
Publication date	Jan 24, 2017
Grant date	Jan 24, 2017

Abstract

A method and a system are provided for detecting particles moving through a detection region or regions for facilitating or processing a sample having one or more particles flowing through the detection region. The particle detection system may include an optically detectable pattern associated with a detection region. The optically detectable pattern may be configured to receive a particle optical signal and produce a patterned optical signal. The detection system may further include a detector configured to analyze the patterned optical signal to determine both a particle characteristic based on a property of the particle optical signal and a particle parameter based on a property of the optically detectable pattern.

First claim

Having described the invention, what is claimed as new and protected by Letters Patent is:

1. A particle detecting system comprising:
a first flow path having a detection region adapted to facilitate analysis or processing of a sample having one or more particles flowing through the first flow path of the detection region;
a first optically detectable pattern associated with the detection region, the first optically detectable pattern positioned to receive a particle optical signal associated with a particle flowing through the first flow path of the detection region and to produce a patterned optical signal; and
a first detector positioned to receive the patterned optical signal,
wherein the first detector is configured to analyze the patterned optical signal to independently determine both:
(i) a particle characteristic based on a property of the particle optical signal associated with the particle flowing through the detection region; and
(ii) a particle parameter based on a property of the first optically detectable pattern.

2. The particle detecting system of claim 1 , wherein the first optically detectable pattern is a gradient masking pattern.

3. The particle detecting system of claim 1 , wherein the first optically detectable pattern includes one or more bars having a width ranging from 10 microns to 30 microns.

4. The particle detecting system of claim 1 , wherein the first optically detectable pattern includes a plurality of bars of varying width.

5. The particle detecting system of claim 1 , wherein the first optically detectable pattern includes a plurality of bars of constant width.

6. The particle detecting system of claim 1 , wherein the first optically detectable pattern includes at least one opening permitting exit of light from or entry of light into the detection region.

7. The particle detecting system of claim 1 , wherein the first flow path is formed at least partially in a substrate and the first optically detectable pattern is deposited on a first side of the substrate.

8. The particle detecting system of claim 1 , wherein the first optically detectable pattern is located between the detection region and a light source.

9. The particle detecting system of claim 1 , wherein the first optically detectable pattern is located between the detection region and the first detector.

10. The particle detecting system of claim 1 , wherein the first optically detectable pattern is located between the first flow path and at least one of a light source and the first detector.

11. The particle detecting system of claim 1 , wherein the first optically detectable pattern is located within the detection region associated with a sorting chip.

12. The particle detecting system of claim 1 , wherein the first optically detectable pattern is used to identify a location of a particle.

13. The particle detecting system of claim 1 , wherein the first optically detectable pattern produces a pattern enabling the detector to identify a location of the first optically detectable pattern.

14. The particle detecting system of claim 1 , wherein the particle characteristic includes one of an optical absorption particle characteristic, a fluorescent intensity particle characteristic or a scattered light particle characteristic.

15. The particle detecting system of claim 1 , wherein the first optically detectable pattern is associated with a first microfluidic flow path.

16. The particle detecting system of claim 1 , wherein the first optically detectable pattern includes a plurality of edges and the first detector is configured to detect the edges.

17. The particle detecting system of claim 1 , wherein the first optically detectable pattern is used to identify both a location particle parameter and a velocity particle parameter of a particle.

18. The particle detecting system of claim 1 ,
wherein the detector is configured to detect at least one of an optical absorption particle characteristic, a fluorescent intensity particle characteristic, or a scattered light particle characteristic for a particle flowing through the first flow path and is configured to detect the first optically detectable pattern associated with the first flow path.

19. The particle detecting system of claim 1 , further comprising:
an actuator for sorting the particle flowing through the detection region into one of a plurality of outlets based on the one or more particle characteristics detected by the first detector.

20. The particle detecting system of claim 1 , wherein the particle parameter includes at least one of a particle velocity, a particle location or a particle size.

21. The particle detecting system of claim 1 , further comprising:
a second flow path; and
a second optically detectable pattern associated with the second flow path.

22. The particle detecting system of claim 21 ,
wherein the detector is configured to independently determine:
at least one of an optical absorption particle characteristic, a fluorescent intensity particle characteristic or a scattered light particle characteristic for a particle flowing through at least one of the first and second flow paths; and
a velocity based on the property of the optically detectable pattern for the same flow path.

23. The particle detecting system of claim 21 , wherein the first or second optically detectable pattern is used to determine at least one of a velocity, a size, or a location of a particle flowing through the first or second flow path, respectively.

24. A method of operating a particle detecting system, the method comprising:
providing the particle detecting system of claim 1 ;
flowing particles through the detection region of the particle detecting system; and
sensing the patterned optical signal exiting from the detection region with the first detector; and
analyzing the patterned optical signal to independently determine both:
(i) a particle characteristic based on a property of the particle optical signal associated with the particle flowing through the detection region; and
(ii) a particle parameter based on a property of the first optically detectable pattern.

25. The method of claim 24 , wherein analyzing the patterned optical signal includes determining at least one of a velocity particle parameter and a location particle parameter associated with the particle within the detection region, and further determining at least one of an optical absorption particle characteristic, a fluorescent intensity particle characteristic and a scattered light particle characteristic associated with the same particle within the detection region.

26. The method of claim 24 , wherein
flowing particles through the detection region includes flowing particles along the first flow path;
sensing the patterned optical signal includes sensing the patterned optical signal exiting from the first flow path;
determining a velocity particle parameter and a location particle parameter from the patterned optical signal; and
determining at least one of an optical absorption particle characteristic, a fluorescent intensity particle characteristic and a scattered light particle characteristic from the same patterned optical signal.

27. The method of claim 26 , further including:
flowing particles through a second flow path included in the detection region;
sensing a second patterned optical signal exiting from the second flow path;

Assignees

• Cytonome St Llc

Inventors

• Deshpande Manish
• Gilbert John R

Classifications

• B07C5/34 Primary

  Sorting according to other particular properties {(material testing per se G01N; quality control G07C3/14)} · CPC title

• F16K99/0019

  Valves using a microdroplet or microbubble as the valve member · CPC title

• G01N2015/1075

  Physics · mapped topic

• B01L3/50273

  characterised by the means or forces applied to move the fluids · CPC title

• Y10S209/932

  Fluid applied to items · CPC title