Microparticle sorting apparatus, microchip for sorting microparticles and microparticle sorting method

Patent metadata

Field	Value
Publication number	US-9448157-B2
Application number	US-201314413543-A
Country	US
Kind code	B2
Filing date	May 31, 2013
Priority date	Jul 18, 2012
Publication date	Sep 20, 2016
Grant date	Sep 20, 2016

Abstract

There is provided a microparticle sorting apparatus including a main channel through which a fluid including microparticles flows, a branch channel that is in communication with the main channel, an actuator that causes a negative pressure to be generated in the branch channel, and a drive unit that controls a voltage applied to the actuator to cause a change in pressure containing a step waveform component and an undershoot waveform component in the branch channel.

First claim

The invention claimed is:

1. A microparticle sorting apparatus, comprising:
a main channel through which a fluid including microparticles flows;
a branch channel that is in communication with the main channel;
a first actuator and a second actuator that cause a negative pressure to be generated in the branch channel, wherein the first actuator and the second actuator are arranged opposite to each other at a position corresponding to the branch channel, and
a drive unit configured to apply different voltages to the first actuator and second actuator to cause a change in pressure in the branch channel,
wherein the drive unit is configured to apply a voltage of a pulse waveform to one of the first actuator and the second actuator, while applying a voltage of a step waveform to other of the first actuator and the second actuator.

2. The microparticle sorting apparatus according to claim 1 , wherein the drive unit is configured to apply the voltage of the pulse waveform to one of the first and second actuators, while applying the voltage of an undershoot-step waveform to other of the first and second actuators.

3. The microparticle sorting apparatus according to claim 1 ,
wherein the main channel and the branch channel are formed inside a microchip, and
wherein the first and second actuators are arranged in contact with a surface of the microchip at a position corresponding to the branch channel.

4. The microparticle sorting apparatus according to claim 3 , wherein the first and second actuators provide displacement to a contact surface of the microchip.

5. The microparticle sorting apparatus according to claim 4 , wherein a portion of the branch channel corresponding to an arrangement position of the first and second actuators is configured as a pressure chamber where a change in volume is caused by the displacement.

6. The microparticle sorting apparatus according to claim 5 ,
wherein in the branch channel, a plurality of pressure chambers are arranged in series, and
wherein the one of the first and second actuators to be applied with the voltage of the pulse waveform is arranged corresponding to a pressure chamber among the plurality of pressure chambers that is located closer to a communication opening to the main channel, than the other of the first and second actuators to be applied with the voltage of the step waveform or the undershoot-step waveform.

7. The microparticle sorting apparatus according to claim 5 , wherein one of the first and second actuators to be applied with the voltage of the pulse waveform is arranged on one surface of the microchip, and other of the first and second actuators to be applied with the voltage of the step waveform or the undershoot-step waveform is arranged on the opposite surface, with respect to the pressure chamber.

8. The microparticle sorting apparatus according to claim 1 , wherein the drive unit is configured to apply a voltage of an undershoot-step waveform to one of the first and second actuators.

9. A microchip for sorting microparticles,
wherein a main channel through which a fluid including microparticles flows, and a branch channel that is in communication with the main channel are formed inside the microchip,
wherein a first actuator and a second actuator that provide displacement on a contact surface, using a drive unit, are arranged opposite to each other at a position corresponding to the branch channel, and
wherein one of the first actuator and the second actuator is applied with a voltage of a pulse waveform using the drive unit, while other of the first actuator and the second actuator is applied with a voltage of a step waveform using the drive unit.

10. The microchip for sorting microparticles according to claim 9 , wherein the branch channel includes a pressure chamber where a change in volume is caused by the displacement.

11. The microchip for sorting microparticles according to claim 10 , further comprising a plurality of combinations of the pressure chamber and the first and second actuators.

12. The microchip for sorting microparticles according to claim 10 , wherein
the first actuator is arranged on one surface of the microchip,
the second actuator is arranged on a surface opposite to the one surface of the microchip, and
the first and second actuators are arranged on the microchip with respect to the pressure chamber.

13. A microparticle sorting method, comprising:
generating a negative pressure within a branch channel that is in communication with a main channel, to draw, into the branch channel, microparticles included in a fluid that flows through the main channel,
wherein in the microparticle sorting method, different voltages are applied to a first actuator and a second actuator to cause a change in pressure in the branch channel, wherein the first actuator and second actuator are arranged opposite to each other at a position corresponding to the branch channel, and
wherein in the microparticle sorting method, a voltage of a pulse waveform is applied to one of the first actuator and the second actuator, while a voltage of a step waveform is applied to other of the first actuator and the second actuator.

14. The microparticle sorting method according to claim 13 , wherein in the microparticle sorting method, a change in pressure of an undershoot-step waveform is caused within the branch channel.

15. The microparticle sorting method according to claim 13 , wherein in the microparticle sorting method,
determining, at a detection area, whether the microparticles in the fluid satisfy predetermined optical characteristics; and
collecting the microparticles that satisfy the predetermined optical characteristics.

Assignees

• Sony Corp

Inventors

• Ito Tatsumi

Classifications

• G01N15/1484 Primary

  microstructural devices · CPC title

• B01L2300/0877

  Flow chambers · CPC title

• B07C5/34

  Sorting according to other particular properties {(material testing per se G01N; quality control G07C3/14)} · CPC title

• B01L2400/0481

  squeezing of channels or chambers · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title