Chlamydomonas mutants produced using RGEN RNP and method for preparing pigment using the same

Patent metadata

Field	Value
Publication number	US-10709152-B2
Application number	US-201816050012-A
Country	US
Kind code	B2
Filing date	Jul 31, 2018
Priority date	Apr 22, 2016
Publication date	Jul 14, 2020
Grant date	Jul 14, 2020

Abstract

The present invention relates to a new alga having an improved ability to produce a pigment, and when a mutant of the present invention is used, a carotenoid-based pigment, specifically, a xanthophyll can be produced by consuming less energy, so that it is possible to efficiently produce the pigment at the industrial level. Further, the pigment can be applied as a raw material for a food, a health functional food and a medicine, which include the pigment. In particular, since a DNA fragment is not likely to be inserted into a target base sequence or a base sequence other than the target, it is expected that the procedure of constructing the mutant is not regulated as a GMO, so that it is expected that the procedure of constructing the mutant can create a big economic effect in terms of an industry which produces lutein and zeaxanthin by using microalgae.

First claim

The invention claimed is:

1. A Chlamydomonas reinhardtii mutant having a ZEP gene mutation in which a base sequence represented by SEQ ID No. 2 is inserted between a 816th base and a 817th base in a ZEP gene sequence of a Chlamydomonas reinhardtii represented by SEQ ID No. 1 and having an ability to produce a xanthophyll.

2. The Chlamydomonas reinhardtii mutant of claim 1 , wherein the Chlamydomonas reinhardtii mutant has a ZEP gene mutation represented by SEQ ID No. 3.

3. The Chlamydomonas reinhardtii mutant of claim 1 , wherein the mutant has an ability to produce one or more pigments selected from a group consisting of lutein and zeaxanthin; and chlorophyll b, chlorophyll a, and β-carotene.

4. A composition for oral administration comprising one or more selected from a group consisting of a culture of the mutant of claim 1 , a dry material thereof, and an extract thereof.

Assignees

• Univ Hanyang Ind Univ Coop Found

Inventors

• Jin Eon Seon
• Bae Sang Su
• Baek Kwang Ryul
• Kim Duk Hyoung
• Jeong Joo Yeon

Classifications

• C12R2001/89

  Algae {; Processes using algae} · CPC title

• C12N1/125

  Unicellular algae isolates · CPC title

• C12Y114/15

  with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15) · CPC title

• C12Y114/1309

  Zeaxanthin epoxidase (1.14.13.90) · CPC title

• C12N1/12

  Unicellular algae; Culture media therefor (as new plants A01H13/00) · CPC title