Anionic exchange-hydrophobic mixed mode

Patent metadata

Field	Value
Publication number	US-10682640-B2
Application number	US-201715581569-A
Country	US
Kind code	B2
Filing date	Apr 28, 2017
Priority date	Mar 8, 2012
Publication date	Jun 16, 2020
Grant date	Jun 16, 2020

Abstract

Solid supports and ligands are provided for purification of biomolecules by mixed-mode anion exchange-hydrophobic chromatography. Compositions can have the formula Support-(X)—N(R1, R2)-R3-L-Ar, or a salt thereof, wherein: Support is a chromatographic solid support; X is a spacer or absent; R1 and R2 are each selected from hydrogen and an alkyl comprising 1-6 carbons; R3 is an alkyl comprising 1-6 carbons or a cyclo alkyl comprising 1-6 carbons; L is NR4, O, or S; wherein R4 is hydrogen or an alkyl comprising 1-6 carbons; and Ar is an aryl. Methods are also provided for using solid supports and ligands to purify biomolecules such as monomeric antibodies.

First claim

What is claimed is:

1. A chromatographic solid support linked to a ligand having the formula:
Support-(X)—N + (R 1 , R 2 )—R 3 -L—Ar, wherein the positively-charged nitrogen is in salt form, and

wherein
X is a spacer or absent;
R 1 and R 2 are each selected from hydrogen and an alkyl comprising 1-6 carbons;
R 3 is an alkyl comprising 1-6 carbons or a cyclo alkyl comprising 1-6 carbons;
L is NR 4 , O, or S; wherein R 4 is hydrogen or an alkyl comprising 1-6 carbons; and
Ar is an aryl.

2. The chromatographic solid support of claim 1 , wherein R 1 and R 2 are hydrogen.

3. The chromatographic solid support of claim 1 , wherein R 3 is an alkyl comprising 1-4 carbons.

4. The chromatographic solid support of claim 1 , wherein R 3 is an alkyl comprising 2 carbons.

5. The chromatographic solid support of claim 1 , wherein the aryl is a phenyl.

6. The chromatographic solid support of claim 1 , wherein the aryl is a substituted phenyl.

7. The chromatographic solid support of claim 6 , wherein the substituted phenyl is an alkyl-substituted phenyl.

8. The chromatographic solid support of claim 1 , wherein R 1 and/or R 2 are an alkyl comprising 1-6 carbons and Ar is an alkyl-substituted phenyl.

9. The chromatographic solid support of claim 1 , wherein the ligand is N-phenylethylenediamine or a salt thereof.

10. The chromatographic solid support of claim 1 , wherein the ligand is 2-phenoxyethylamine or a salt thereof.

11. The chromatographic solid support of claim 1 , wherein X is absent.

12. The chromatographic solid support of claim 1 , wherein X is a spacer.

Assignees

• Bio Rad Laboratories

Inventors

• Liao Jiali
• Frost Russell

Classifications

• B01J20/289

  bonded via a spacer · CPC title

• B01J20/288

  Polar phases · CPC title

• B01J20/286

  Phases chemically bonded to a substrate, e.g. to silica or to polymers · CPC title

• B01J20/28016

  Particle form · CPC title

• B01J41/09

  Organic material · CPC title