Method of purifying a biological material of interest in a sample using nanofiber ultrafiltration membranes operated in tangential flow filtration mode

Patent metadata

Field	Value
Publication number	US-10675588-B2
Application number	US-201615561579-A
Country	US
Kind code	B2
Filing date	Feb 17, 2016
Priority date	Apr 17, 2015
Publication date	Jun 9, 2020
Grant date	Jun 9, 2020

Abstract

Embodiments described herein relate to electrospun nanofiber ultrafiltration membrane compositions capable of operating in tangential filtration mode and methods of using the same.

First claim

What is claimed is:

1. Method of purifying a biological material of interest in a sample comprising:
a. providing a sample containing the biological material of interest having a molecular weight equal to or greater than 500 K Da, wherein the biological material of interest is selected from a therapeutic protein, a conjugated polysaccharide vaccine and a virus-like particle;
b. contacting the sample in tangential filtration (TFF) mode with an electrospun nanofiber membrane composition comprising nylon nanofibers having an average fiber diameter less than 15 nm, thereby resulting in a permeate and a retentate; and
c. collecting the retentate containing the biological material of interest, thereby purifying the biological material of interest.

2. The method of claim 1 , wherein the method results in an increase in the concentration of the biological material of interest.

3. The method of claim 1 , wherein the electrospun nanofiber composition exhibits a higher water permeability in TFF mode compared to a solution cast polymeric membrane.

4. The method of claim 1 , wherein the electrospun nanofiber membrane composition exhibits a higher flux in TFF mode compared to a solution cast polymeric membrane.

5. The method of claim 1 , wherein the collecting step comprises diafiltration.

6. The method of claim 1 , wherein the electrospun nanofiber membrane composition is made from Nylon-6.

7. The method of claim 1 , wherein the purified biological material of interest has a 90% yield or greater than 90% yield.

8. The method of claim 1 , wherein the electrospun nanofiber membrane composition is incorporated into a device suitable for TFF.

9. The method of claim 8 , wherein the device is a cassette or a spiral wound device.

Assignees

• Emd Millipore Corp

Inventors

• Cataldo William
• Kozlov Mikahil
• Xenopoulos Alex
• Aquino Dennis
• Ngan Clifton

Classifications

• B01D71/54

  Polyureas; Polyurethanes · CPC title

• B01D2239/025

  comprising nanofibres · CPC title

• B01D61/145 Primary

  Ultrafiltration · CPC title

• B01D2315/16

  Diafiltration · CPC title

• B01D67/0004

  by agglomeration of particles · CPC title