Method for Retrovirus Removal

Patent metadata

Field	Value
Publication number	US-2017173511-A1
Application number	US-201715448248-A
Country	US
Kind code	A1
Filing date	Mar 2, 2017
Priority date	Aug 10, 2010
Publication date	Jun 22, 2017
Grant date	—

Abstract

A method for removing retroviruses from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention is disclosed. Retroviruses are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having a retrovirus LRV greater than about 6, and the nanofiber(s) has a diameter from about 10 nm to about 100 nm. The filtration medium can be in the form of a fibrous electrospun polymeric nanofiber liquid filtration medium mat.

First claim

What is claimed is:

1 . A porous nanofiber filtration medium for removing retroviruses from a liquid sample, resulting in the full retention of retroviruses, comprising:
a porous nanofiber filtration medium having a retrovirus Log Reduction Value (LRV) greater than about 6, a porosity from about 80% to about 95%, and a liquid permeability greater than about 100 LMH/psi, wherein the nanofiber has a fiber diameter from about 10 nm to about 100 nm.

2 . The medium of claim 1 , further comprising a mean flow bubble point, as tested with isopropanol, from about 100 to about 150 psi.

3 . The medium of claim 1 , having a thickness from about 1 um to about 500 um.

4 . The medium of claim 1 , having a thickness from about 1 um to about 50 um.

5 . The medium of claim 1 , formed by a process selected from the group consisting of electrospinning and electroblowing.

6 . The medium of claim 1 , wherein the retrovirus is Human Immunodeficiency Virus (HIV).

7 . The medium of claim 1 , wherein the retrovirus is Human T-cell leukemia virus (HTLV).

8 . The medium of claim 1 , wherein the nanofiber comprises a polymer selected from the group consisting of polyimide, aliphatic polyamide, aromatic polyamide, polysulfone, cellulose acetate, polyether sulfone, polyurethane, poly(urea urethane), polybenzimidazole, polyetherimide, polyacrylonitrile, poly(ethylene terephthalate), polypropylene, polyaniline, poly(ethylene oxide), poly(ethylene naphthalate), poly(butylene terephthalate), styrene butadiene rubber, polystyrene, poly(vinyl chloride), poly(vinyl alcohol), poly(vinylidene fluoride), poly(vinyl butylene) and copolymers, derivative compounds, or blends thereof.

9 . The medium of claim 1 , wherein the nanofiber comprises an aliphatic polyamide.

10 . The medium of claim 1 , wherein the nanofiber comprises a blend of polymers or copolymers.

11 . The medium of claim 1 , wherein the nanofiber is disposed on a porous support.

12 . The medium of claim 11 , wherein the porous support comprises one or more layers selected from the group consisting of spunbonded nonwovens, meltblown nonwovens, needle punched nonwovens, spunlaced nonwovens, wet laid nonwovens, resin-bonded nonwovens, woven fabrics, knit fabrics, paper, and combinations thereof.

13 . A liquid filtration device having a retrovirus LRV greater than about 6 for use in fully retaining retroviruses removed from a liquid sample passing through the device, the filtration device comprising:
a porous electrospun nanofiber filtration medium having a LRV greater than 6, a porosity from about 80% to about 95%, and a liquid permeability greater than about 100 LMH/psi, and a porous support, wherein the nanofiber filtration medium is disposed on the porous support
and the nanofiber has a fiber diameter from about 10 nm to about 100 nm.

14 . The device of claim 13 , wherein the electrospun nanofiber filtration medium comprises a thickness from about 10 um to about 100 um.

15 . The device of claim 13 , wherein the electrospun nanofiber filtration medium comprises a thickness from 1 μm to 50 μm.

16 . The device of claim 13 , further comprising a mean flow bubble point, as tested with isopropanol, from about 100 to about 150 psi.

17 . The device of claim 13 , wherein the electrospun nanofiber filtration medium comprises a mean pore size from about 0.05 um to about 3 um.

18 . The device of claim 13 , wherein the electrospun nanofiber filtration medium is a polymeric fibrous mat.

19 . The device of claim 18 , wherein the nanofiber comprises a polymer selected from the group consisting of polyimide, aliphatic polyamide, aromatic polyamide, polysulfone, cellulose acetate, polyether sulfone, polyurethane, poly(urea urethane), polybenzimidazole, polyetherimide, polyacrylonitrile, poly(ethylene terephthalate), polypropylene, polyaniline, poly(ethylene oxide), poly(ethylene naphthalate), poly(butylene terephthalate), styrene butadiene rubber, polystyrene, poly(vinyl chloride), poly(vinyl alcohol), poly(vinylidene fluoride), poly(vinyl butylene) and copolymers, derivative compounds, or blends thereof.

20 . The device of claim 13 , wherein the nanofiber comprises an aliphatic polyamide.

21 . The device of claim 13 , wherein the nanofiber comprises a blend of polymers or copolymers.

22 . The device of claim 13 , wherein the nanofiber is disposed on a porous support.

23 . The device of claim 13 , wherein the porous support comprises one or more layers selected from the group consisting of spunbonded nonwovens, meltblown nonwovens, needle punched nonwovens, spunlaced nonwovens, wet laid nonwovens, resin-bonded nonwovens, woven fabrics, knit fabrics, paper, and combinations thereof.

24 . The device of claim 13 , wherein the retrovirus is retrovirus is Human Immunodeficiency Virus (HIV).

25 . The device of claim 13 , wherein the retrovirus is Human T-cell leukemia virus (HTLV).

Assignees

• Emd Millipore Corp

Inventors

• Kas Onur Y
• Kozlov Mikhail
• Moya Wilson
• Leon Sherry A
• Goddard Philip
• Hao Jibin
• Tkacik Gabriel

Classifications

• B01D39/1615

  of natural origin · CPC title

• B01D2239/0631

  Electro-spun · CPC title

• B01D2325/20

  Specific permeability or cut-off range · CPC title

• B01D69/02

  characterised by their properties · CPC title

• B01D39/04

  Organic material, e.g. cellulose, cotton · CPC title