Method for retrovirus removal

Patent metadata

Field	Value
Publication number	US-9623352-B2
Application number	US-201113194227-A
Country	US
Kind code	B2
Filing date	Jul 29, 2011
Priority date	Aug 10, 2010
Publication date	Apr 18, 2017
Grant date	Apr 18, 2017

Abstract

A method for removing retroviruses from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention is disclosed. Retroviruses are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having a retrovirus LRV greater than about 6, and the nanofiber(s) has a diameter from about 10 nm to about 100 nm. The filtration medium can be in the form of a fibrous electrospun polymeric nanofiber liquid filtration medium mat.

First claim

What is claimed is:

1. A method of removing retroviruses from a liquid sample comprising the steps of:
a) providing a liquid sample containing retroviruses,
b) providing a filtration medium comprising a porous nanofiber mat made by electrospinning a polymer, wherein the porous nanofiber mat has a retrovirus Log Reduction Value (LRV) greater than about 6, a porosity from about 80% to about 95%, and a liquid permeability greater than about 100 LMH/psi, and wherein the nanofiber mat has a fiber diameter effective for retention of retroviruses by size-based exclusion, and
c) passing the liquid sample containing the retroviruses through the filtration medium such that the retroviruses are retained by the filtration medium.

2. The method of claim 1 , wherein the porous nonofiber mat exhibits a mean flow bubble point, as tested with isopropanol, from 100 to 150 psi.

3. The method of claim 1 , wherein the filtration medium has a thickness from about 1 μm to about 500 μm.

4. The method of claim 1 , wherein the porous nonofiber mat has a thickness from 1 μm to 50 μm.

5. The method of claim 1 , wherein the retrovirus is Human Immunodeficiency Virus (HIV).

6. The method of claim 1 , wherein the retrovirus is Human T-cell leukemia virus (HTLV).

7. The method of claim 1 , wherein the polymer is selected from the group consisting of polyimide, aliphatic polyamide, aromatic polyamide, polysulfone, cellulose acetate, polyether sulfone, polyurethane, poly(urea urethane), polybenzimidazole, polyetherimide, polyacrylonitrile, poly(ethylene terephthalate), polypropylene, polyaniline, poly(ethylene oxide), poly(ethylene naphthalate), poly(butylene terephthalate), styrene butadiene rubber, polystyrene, poly(vinyl chloride), poly(vinyl alcohol), poly(vinylidene fluoride), poly(vinyl butylene) and copolymers, derivative compounds, or blends thereof.

8. The method of claim 1 , wherein the polymer comprises an aliphatic polyamide.

9. The method of claim 1 , wherein the polymer comprises a blend of polymers or copolymers.

10. The method of claim 1 , wherein the porous nanofiber mat is disposed on a porous support.

11. The method of claim 10 , wherein the porous support comprises one or more layers selected from the group consisting of spunbonded nonwovens, meltblown nonwovens, needle punched nonwovens, spunlaced nonwovens, wet laid nonwovens, resin-bonded nonwovens, woven fabrics, knit fabrics, paper, and combinations thereof.

12. The method of claim 1 , wherein the porous nanofiber mat has a fiber diameter from about 25-30 nm.

13. The method of claim 1 , wherein the porous nanofiber mat has a fiber diameter from about 10-100 nm.

14. The method of claim 1 , wherein the porous nanofiber mat has a liquid permeability of at least about 300 LMH/psi.

15. The method of claim 2 , wherein the porous nanofiber mat has a fiber diameter from about 25-30 nm.

16. The method of claim 2 , wherein the porous nanofiber mat has a fiber diameter from about 10-100 nm.

17. The method of claim 2 , wherein the porous nanofiber mat has a liquid permeability of at least about 300 LMH/psi.

18. The method of claim 4 , wherein the porous nanofiber mat has a fiber diameter from about 25-30 nm.

19. The method of claim 4 , wherein the porous nanofiber mat has a fiber diameter from about 10-100 nm.

20. The method of claim 4 , wherein the porous nanofiber mat has a liquid permeability of at least about 300 LMH/psi.

Assignees

• Kas Onur Y
• Kozlov Mikhail
• Moya Wilson
• Goddard Philip
• Leon Sherry Ashby
• Hao Jibin
• Tkacik Gabriel
• Emd Millipore Corp

Inventors

• Kas Onur Y
• Kozlov Mikhail
• Moya Wilson
• Goddard Philip
• Leon Sherry Ashby
• Hao Jibin
• Tkacik Gabriel

Classifications

• D01D5/0092

  characterised by the electrical field, e.g. combined with a magnetic fields, using biased or alternating fields · CPC title

• B01D39/1615

  of natural origin · CPC title

• B01D2239/10

  Filtering material manufacturing · CPC title

• B01D67/002

  from melts · CPC title

• A61L2/022 Primary

  Filtration · CPC title