Composition for stimulating insulin secretion from cells and use thereof

Patent metadata

Field	Value
Publication number	US-10653732-B2
Application number	US-201815935634-A
Country	US
Kind code	B2
Filing date	Mar 26, 2018
Priority date	Mar 27, 2017
Publication date	May 19, 2020
Grant date	May 19, 2020

Abstract

Provided are a composition for stimulating insulin secretion from cells, the composition including a Hypoxylon truncatum extract or an active ingredient thereof, and a method of stimulating insulin secretion from cells.

First claim

What is claimed is:

1. A method of stimulating insulin secretion from cells, the method comprising contacting a composition or an extract with cells,
wherein the composition comprises any stereoisomer selected from the group consisting of Formulae I, II, and III, a solvate or hydrate thereof, or a mixture thereof, or a physiologically acceptable salt thereof:

wherein, in Formula I, II, and III, R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 are each independently H or (C 1 -C 6 )-alkyl, and
the extract is a Hypoxylon sp. mushroom extract comprising any stereoisomer selected from the group consisting of Formulae I, II, and III, a solvate or hydrate thereof, or a mixture thereof, or a physiologically acceptable salt thereof:

wherein, in Formula I, II, and III, R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 are each independently H or (C 1 - C 6 ) alkyl.

2. The method of claim 1 , wherein the contacting is to administer the composition or the extract to a subject.

3. The method of claim 1 , wherein the cells are pancreatic beta cells.

4. The method of claim 1 , wherein the method is to prevent or treat diabetes in the subject.

5. The method of claim 1 , wherein R 1 and R 5 are each independently H or (C 1 -C 6 )-alkyl, and R 2 , R 3 , R 4 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 are each independently H.

6. The method of claim 5 , wherein R 1 and R 5 are each independently H, methyl, or ethyl.

7. The method of claim 1 , wherein the compounds of Formula I, II, and III exist at a weight ratio of 1.0: 0.20 to 0.60: 0.50 to 1.00 in the composition.

8. The method of claim 1 , the composition further comprising an excipient or a carrier which is acceptable for use in pharmaceuticals or foods.

9. The method of claim 5 , wherein the composition is a pharmaceutical composition or a food composition.

10. The method of claim 4 , wherein the diabetes is type 1 or type 2 diabetes.

11. The method of claim 1 , the composition comprising a Hypoxylon sp. mushroom extract comprising any stereoisomer selected from the group consisting of Formulae I, II, and III, or a mixture thereof, or a physiologically acceptable salt thereof.

12. The method of claim 11 , wherein the extract is extracted by an extraction method comprising contacting Hypoxylon sp. mushroom with water, (C 1 -C 3 )-alcohol, or a mixture thereof.

13. The method of claim 12 , wherein the extract is obtained by fractionating the extract with a R 13 —COO—R 14 solvent, wherein R 13 and R 14 are each independently (C 1 -C 6 )-alkyl.

14. The method of claim 12 , wherein the extract is a fraction obtained by fractionating the extract with a (C 4 -C 12 )-alkane solvent to obtain a fraction, removing this fraction to obtain a residue, fractionating the residue with a R 13 —COO—R 14 solvent, wherein R 13 and R 14 is each independently (C 1 -C 6 )-alkyl, thereby obtained the fraction.

Assignees

• Korea Inst Sci & Tech

Inventors

• Ham Jungyeob
• Chung Bong Chul
• Kim Taejung
• Choi Pilju
• Kang Ki Sung
• Hwang Buyng Su
• Kim Kyu Sun

Classifications

• A61K36/062 Primary

  Ascomycota · CPC title

• A23L33/10

  using additives (addition of substantially indigestible substances A23L33/21) · CPC title

• A61P3/10

  for hyperglycaemia, e.g. antidiabetics · CPC title

• A61K2236/39

  Complex extraction schemes, e.g. fractionation or repeated extraction steps · CPC title

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title