Extracts from fusarium oxysporum and use thereof

Patent metadata

Field	Value
Publication number	US-9950020-B2
Application number	US-201314384898-A
Country	US
Kind code	B2
Filing date	Feb 1, 2013
Priority date	Mar 13, 2012
Publication date	Apr 24, 2018
Grant date	Apr 24, 2018

Abstract

Disclosed are the action and use of water extracts and polysaccharides from Fusarium oxysporum in immune enhancement, which belong to the field of biological medicine. The water extracts and polysaccharides from Fusarium oxysporum have a significant immune enhancement effect, which can promote the proliferation of macrophages, increase the phagocytic ability of macrophages, activate macrophages to release immunological active factors NO, IL-1α and TNF-α and can be used in lower immune function diseases and tumor adjuvant treatment.

First claim

What is claimed is:

1. A method for preparing an immune enhancement drug comprising the steps of:
(a) cultivating Fusarium oxysporum with a liquid shake flask culture and fermentation tank culture to obtain a fungus leavening;
(b) centrifuging the fungus leavening to obtain mycelia; and
(c) extracting the mycelia to obtain the water extracts and/or the crude polysaccharides.

2. A method for preparing a drug for treating lower immune function diseases comprising the steps of:
(a) cultivating Fusarium oxysporum with a liquid shake flask culture and fermentation tank culture to obtain a fungus leavening;
(b) centrifuging the fungus leavening to obtain mycelia; and
(c) extracting the mycelia to obtain water extracts and/or crude polysaccharides.

3. A method for preparing a drug for tumor adjuvant treatment comprising the steps of:
(a) cultivating Fusarium oxysporum with a liquid shake flask culture and fermentation tank culture to obtain a fungus leavening;
(b) centrifuging the fungus leavening to obtain mycelia; and
(c) extracting the mycelia to obtain water extracts and/or crude polysaccharides.

4. A method for preparing an immune enhancement drug comprising Fusarium oxysporum , and the steps of:
(a) cultivating Fusarium oxysporum with a liquid shake flask culture and fermentation tank culture to obtain a fungus leavening;
(b) centrifuging the fungus leavening to obtain mycelia; and
(c) extracting the mycelia to obtain water extracts and/or crude polysaccharides.

5. A method for preparing a drug for treating lower immune function diseases comprising Fusarium oxysporum , and the steps of:
(a) cultivating Fusarium oxysporum with a liquid shake flask culture and fermentation tank culture to obtain a fungus leavening;
(b) centrifuging the fungus leavening to obtain mycelia; and
(c) extracting the mycelia to obtain water extracts and/or crude polysaccharides.

6. A method for preparing a drug for tumor adjuvant treatment comprising Fusarium oxysporum , a water extract and/or crude polysaccharides from Fusarium oxysporum , the method comprising the steps of:
(a) cultivating Fusarium oxysporum with a liquid shake flask culture and fermentation tank culture to obtain a fungus leavening;
(b) centrifuging the fungus leavening to obtain mycelia; and
(c) extracting the mycelia to obtain the water extract and/or the crude polysaccharides.

7. The method according to claim 1 , wherein the step (c) of extracting the mycelia to obtain the water extract and/or the crude polysaccharides further comprises:
(d) drying and crushing the mycelia to provide a product;
(e) extracting the product of step (d) with a hot reflux extraction to obtain an extracting solution;
(f) concentrating and drying the extracting solution to obtain the water extract;
(g) optionally, concentrating and precipitating the extracting solution with an to provide an alcohol product;
(h) centrifuging the alcohol product of step (g) to obtain a sediment; and
(i) drying the sediment to obtain the crude polysaccharides.

8. The method according to claim 2 , wherein the step (c) of extracting the mycelia to obtain the water extract and/or the crude polysaccharides further comprises:
(d) drying and crushing the mycelia to provide a product;
(e) extracting the product of step (d) with a hot reflux extraction to obtain an extracting solution;
(f) concentrating and drying the extracting solution to obtain the water extract;
(g) optionally, concentrating and precipitating the extracting solution with an to provide an alcohol product;
(h) centrifuging the alcohol product of step (g) to obtain a sediment; and
(i) drying the sediment to obtain the crude polysaccharides.

9. The method according to claim 3 , wherein the step (c) of extracting the mycelia to obtain the water extract and/or the crude polysaccharides further comprises:
(d) drying and crushing the mycelia to provide a product;
(e) extracting the product of step (d) with a hot reflux extraction to obtain an extracting solution;
(f) concentrating and drying the extracting solution to obtain the water extract;
(g) optionally, concentrating and precipitating the extracting solution with an to provide an alcohol product;
(h) centrifuging the alcohol product of step (g) to obtain a sediment; and
(i) drying the sediment to obtain the crude polysaccharides.

10. The method according to claim 4 , wherein the step (c) of extracting the mycelia to obtain the water extracts and/or the crude polysaccharides further comprises:
(d) drying and crushing the mycelia to provide a product;
(e) extracting the product of step (d) with a hot reflux extraction to obtain an extracting solution;
(f) concentrating and drying the extracting solution to obtain the water extract;
(g) optionally, concentrating and precipitating the extracting solution with an to provide an alcohol product;
(h) centrifuging the alcohol product of step (g) to obtain a sediment; and
(i) drying the sediment to obtain the crude polysaccharides.

11. The method according to claim 5 , wherein the step (c) of extracting the mycelia to obtain the water extract and/or the crude polysaccharides further comprises:
(d) drying and crushing the mycelia to provide a product;
(e) extracting the product of step (d) with a hot reflux extraction to obtain an extracting solution;
(f) concentrating and drying the extracting solution to obtain the water extract;
(g) optionally, concentrating and precipitating the extracting solution with an to provide an alcohol product;
(h) centrifuging the alcohol product of step (g) to obtain a sediment; and
(i) drying the sediment to obtain the crude polysaccharides.

12. The method according to claim 6 , wherein the step (c) of extracting the mycelia to obtain the water extract and/or the crude polysaccharides further comprises:
(d) drying and crushing the mycelia to provide a product;
(e) extracting the product of step (d) with a hot reflux extraction to obtain an extracting solution;
(f) concentrating and drying the extracting solution to obtain the water extract;
(g) optionally, concentrating and precipitating the extracting solution with an to provide an alcohol product;
(h) centrifuging the alcohol product of step (g) to obtain a sediment; and
(i) drying the sediment to obtain the crude polysaccharides.

Assignees

• Univ Macau

Inventors

• Li Shaoping
• Feng Kun
• Meng Lanzhen
• Zhao Jing

Classifications

• A61P35/00

  Antineoplastic agents · CPC title

• A61P37/04

  Immunostimulants · CPC title

• A61K36/062 Primary

  Ascomycota · CPC title

• A61K35/74

  Bacteria (therapeutic use of a bacterial protein A61K38/00) · CPC title