Agricultural microbial inoculant compositions and uses thereof

Patent metadata

Field	Value
Publication number	US-10513467-B2
Application number	US-201715702417-A
Country	US
Kind code	B2
Filing date	Sep 12, 2017
Priority date	Jun 2, 2015
Publication date	Dec 24, 2019
Grant date	Dec 24, 2019

Abstract

The present disclosure provides novel agricultural microbial inoculant compositions for uses in promoting plant growth, plant productivity and/or soil quality. The novel microbial inoculant compositions comprise one or more microbial species, one or more urease inhibitors and/or one or more nitrification inhibitors. The present disclosure also provides fertilizer compositions comprising said microbial inoculant compositions.

First claim

What is claimed is:

1. A composition comprising:
i. a Bacillus species selected from the group consisting of Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus thuringiensis, Bacillus pumilus , and mixtures thereof;
ii. an organic solvent selected from the group consisting of propylene glycol (PG), N-methyl-2-pyrrolidone (NMP), triethylene glycol monobutyl ether, glycerol, dimethyl sulfoxide (DMSO), and mixtures thereof, wherein the organic solvent is present in an amount ranging from 20 weight percent to 99 weight percent, based on the total weight of the composition; and
iii. a urease inhibitor selected from the group consisting of N-(n-butyl)thiophosphoric triamide (NBPT), N-(n-butyl)phosphoric triamide, thiophosphoryl triamide, phenyl phosphorodiamidate, N-cyclohexyl phosphoric triamide, N-cyclohexyl thiophosphoric triamide, phosphoric triamide, hydroquinone, p-benzoquinone, hexamidocyclotriphosphazene, thiopyridines, thiopyrimidines, thiopyridine-N-oxides, N,N-dihalo-2-imidazolidinone, N-halo-2-oxazolidinone, N-(2-nitrophenyl)thiophosphoric triamide, N-(2-nitrophenyl)phosphoric triamide, and mixtures thereof.

2. The composition of claim 1 , wherein the Bacillus species is Bacillus licheniformis, Bacillus thuringiensis , or a combination thereof.

3. The composition of claim 1 , wherein the Bacillus species is selected from the group consisting of Bacillus amyloliquefaciens ATCC 23842 , Bacillus licheniformis ATCC 14580 , Bacillus licheniformis B-642 , Bacillus licheniformis B-14368 , Bacillus thuringiensis ATCC 10792 , Bacillus thuringiensis HD-17 , Bacillus thuringiensis HD-1, and Bacillus pumilus NRS-272.

4. The composition of claim 1 , wherein the urease inhibitor is N-(n-butyl)thiophosphoric triamide (NBPT).

5. The composition of claim 1 , further comprising a nitrification inhibitor selected from the group consisting of 2-chloro-6-trichloromethyl pyridine, 5-ethoxy-3-trichloromethyl-1,2,4-thiadiazol, dicyandiamide, 2-amino-4-chloro-6-methyl-pyrimidine, 1,3-benzothiazole-2-thiol, 4-amino-N-1,3-thiazol-2-ylbenzene sulfonamide, thiourea, guanidine, 3,4-dimethylpyrazole phosphate, 2,4-diamino-6- trichloromethyl-5-triazine, poly etherionophores, 4-amino-1,2,4-triazole, 3-mercapto-1,2,4- triazole, potassium azide, carbon bisulfide, sodium trithiocarbonate, ammonium dithiocarbamate, 2,3,-dihydro-2,2-dimethyl-7-benzofuranol methylcarbamate, N-(2,6-dimethylphenyl)-N(methoxyacetyl)-alanine methyl ester, ammonium thiosulfate, 1-hydroxypyrazole, 3-methylpyrazole-1-carboxamide, 3-methylpyrazole, 3,5-dimethylpyrazole, 1,2,4-triazole, G77 Nitrification Inhibitor (CAS Registration No. 1373256-33-7), and mixtures thereof.

6. The composition of claim 5 , wherein the nitrification inhibitor is dicyandiamide, G77 Nitrification Inhibitor (CAS Registration No. 1373256-33-7), or a combination thereof.

7. The composition of claim 1 , wherein the urease inhibitor is present in an amount ranging from 10 weight % to 80 weight % based on the total weight of the composition.

8. The composition of claim 1 , wherein the Bacillus species is viable for at least 2 hours from the time when said Bacillus species contacts said organic solvent.

9. The composition of claim 1 , wherein the Bacillus species concentration is in the range of from 1.0×10 2 spores/mL to 1.0×10 12 spores/mL.

10. The composition of claim 1 , further comprising a dye.

11. The composition of claim 1 , comprising propylene glycol and N-methyl-2-pyrrolidone.

12. The composition of claim 11 , wherein the propylene glycol is present in an amount ranging from 40 weight % to 70 weight % based on the total weight of the composition, the N-methyl-2-pyrrolidone is present in an amount ranging from 15 weight % to 40 weight % based on the total weight of the composition, the urease inhibitor is NBPT, and the-NBPT is present in an amount ranging from 10 weight % to 30 weight % based on the total weight of the composition.

13. A fertilizer composition comprising the composition according to claim 1 , and a nitrogen source.

14. A method for promoting plant growth, plant productivity and/or soil quality, wherein the method comprises applying an effective amount of the composition according to claim 1 to a plant, plant part, plant seed, or soil.

15. A kit comprising:
Part A, wherein Part A comprises a urease inhibitor and an organic solvent,
wherein said urease inhibitor is selected from the group consisting of N-(n-butyl)thiophosphoric triamide (NBPT), N-(n-butyl)phosphoric triamide, thiophosphoryl triamide, phenyl phosphorodiamidate, N-cyclohexyl phosphoric triamide, N-cyclohexyl thiophosphoric triamide, phosphoric triamide, hydroquinone, p-benzoquinone, hexamidocyclotriphosphazene, thiopyridines, thiopyrimidines, thiopyridine-N-oxides, N,N-dihalo-2-imidazolidinone, N-halo-2-oxazolidinone, N-(2-nitrophenyl)thiophosphoric triamide, N-(2-nitrophenyl)phosphoric triamide, and mixtures thereof; and
wherein said organic solvent is selected from the group consisting of propylene glycol (PG), N-methyl-2-pyrrolidone (NMP), triethylene glycol monobutyl ether, glycerol, dimethyl sulfoxide (DMSO), and mixtures thereof; and

Part B, wherein Part B comprises a Bacillus species selected from the group consisting of Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus thuringiensis, Bacillus pumilus , and mixtures thereof,
wherein each of Part A and Part B is contained in a separate container, and
wherein the organic solvent is present in an amount ranging from 20 weight % to 99 weight %, based on the combined weight of Part A and Part B.

16. The kit of claim 15 , wherein Part B comprises a Bacillus species selected from the group consisting of Bacillus licheniformis and Bacillus thuringiensis.

17. The kit of claim 15 , wherein Part B comprises a Bacillus strain selected from the group consisting of Bacillus amyloliquefaciens (ATCC 23842), Bacillus licheniformis (ATCC 14580), Bacillus licheniformis (B-642), Bacillus licheniformis (B-14368), Bacillus thuringiensis (ATCC 10792), Bacillus thuringiensis (HD-17), Bacillus thuringiensis (HD-1), and Bacillus pumilus (NRS-272).

18. The kit of claim 15 , wherein the urease inhibitor is N-(n-butyl)thiophosphoric triamide (NBPT).

19. The kit of claim 18 , wherein Part A comprises propylene glycol and N-methyl-2-pyrrolidone.

20. The kit of claim 19 , wherein the propylene glycol is present in an amount ranging from 40 weight % to 70 weight % based on the total weight of Part A, the N-methyl-2-pyrrolidone is present in an amount ranging from 15 weight % to 40 weight % based on the total weight of Part A, and the NBPT is present in an amount ranging from 10 weight % to 30 weight % based on the total weight of Part A.

21. The kit of claim 15 , wherein the Bacillus species is viable for at least 2 hours from the time when said Bacillus species in Part A contacts said organic solvent in Part B.

Assignees

• Koch Agronomic Services Llc

Inventors

• Bobeck Drew R
• Pearce Cedric J

Classifications

• C05G3/90

  for affecting the nitrification of ammonium compounds or urea in the soil · CPC title

• C05G3/60

  Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants · CPC title

• C05F11/08 Primary

  Organic fertilisers containing added bacterial cultures, mycelia or the like · CPC title

• C05G3/00

  Mixtures of one or more fertilisers with additives not having a specially fertilising activity · CPC title

• A01N25/04

  Dispersions, {emulsions, suspoemulsions, suspension concentrates} or gels (foams A01N25/16) · CPC title