Disease resistant potato plants

Patent metadata

Field	Value
Publication number	US-10501754-B2
Application number	US-201615191919-A
Country	US
Kind code	B2
Filing date	Jun 24, 2016
Priority date	Feb 1, 2007
Publication date	Dec 10, 2019
Grant date	Dec 10, 2019

Abstract

The present invention relates to a plant, which is resistant to a pathogen of viral, bacterial, fungal or oomycete origin, wherein the plant has a reduced level, reduced activity or complete absence of DMR6 protein as compared to a plant that is not resistant to the said pathogen, in particular organisms of the Fungi or the phylum Oomycota. The invention further relates to a method for obtaining a plant, which is resistant to a pathogen of viral, bacterial, fungal or oomycete origin, comprising reducing the endogenous level or activity of DMR6 protein in the plant. In addition, the invention relates to the use of a DMR6 promotor for providing disease resistant plants.

First claim

The invention claimed is:

1. An isolated potato plant which is resistant to Phytophthora infestans , wherein the potato plant has reduced expression of a first DMR6 protein of SEQ ID NO: 112 as compared to a corresponding wild-type potato plant, and wherein the potato plant has a non-natural mutation introduced into a first dmr6 gene of SEQ ID NO: 113 encoding said first DMR6 protein; and
wherein the potato plant has reduced expression of a second DMR6 protein of SEQ ID NO: 114 as compared to a corresponding wild-type potato plant, and wherein the potato plant has a non-natural mutation introduced into a second dmr6 gene of SEQ ID NO: 115 encoding said second DMR6 protein.

2. The potato plant as claimed in claim 1 , wherein the mutation in the first dmr6 gene leads to an amino acid substitution in the first DMR6 protein and the mutation in the second dmr6 gene leads to an amino acid substitution in the second DMR6 protein.

3. The potato plant as claimed in claim 1 , wherein the potato plant has a non-natural mutation introduced into the first dmr6 gene of SEQ ID NO: 113 and the potato plant has reduced expression of the first DMR6 protein of SEQ ID NO: 112.

4. A seed, tissue, or plant part of the potato plant according to claim 1 , wherein the seed, tissue, or plant part comprises the mutation in the first dmr6 gene of SEQ ID NO: 113 and has reduced expression of the first DMR6 protein of SEQ ID NO: 112.

5. The potato plant as claimed in claim 1 , wherein the potato plant has a non-natural mutation introduced into the second dmr6 gene of SEQ ID NO: 115 and the potato plant has reduced expression of the second DMR6 protein of SEQ ID NO: 114.

6. A seed, tissue, or plant part of the potato plant according to claim 1 , wherein the seed, tissue, or plant part comprises the mutation in the second dmr6 gene of SEQ ID NO: 115 and has reduced expression of the second DMR6 protein of SEQ ID NO: 114.

7. A method for obtaining a potato plant which is resistant to Phytophthora infestans , comprising reducing an endogenous level of a first DMR6 protein of SEQ ID NO: 112 in a potato plant by introducing a mutation into a first dmr6 gene of SEQ ID NO: 113 encoding said first DMR6 protein, and reducing an endogenous level of a second DMR6 protein of SEQ ID NO: 114 in a potato plant by introducing a mutation into a second dmr6 gene of SEQ ID NO: 115 encoding said second DMR6 protein.

8. The method according to claim 7 , wherein the endogenous level of the first DMR6 protein of SEQ ID NO: 112 in the potato plant is reduced by introducing a mutation into the first dmr6 gene of SEQ ID NO: 113.

9. The method according to claim 7 , wherein reducing the endogenous level of the first DMR6 protein of SEQ ID NO: 112 in the potato plant is achieved by reducing expression of the first dmr6 gene of SEQ ID NO: 113.

10. The method according to claim 9 , wherein reducing expression of the first dmr6 gene of SEQ ID NO: 113 is achieved by gene silencing or RNAi.

11. The method according to claim 7 , wherein the mutation results in an amino acid substitution in the first DMR6 protein of SEQ ID NO: 112.

12. The method according to claim 7 , wherein the endogenous level of the second DMR6 protein of SEQ ID NO: 114 in the potato plant is reduced by introducing a mutation into the second dmr6 gene of SEQ ID NO: 115.

13. The method according to claim 7 , wherein reducing the endogenous level of the second DMR6 protein of SEQ ID NO: 114 in the potato plant is achieved by reducing expression of the second dmr6 gene of SEQ ID NO: 115.

14. The method according to claim 13 , wherein reducing expression of the second dmr6 gene of SEQ ID NO: 115 is achieved by gene silencing or RNAi.

15. The method according to claim 7 , wherein the mutation results in an amino acid substitution in the second DMR6 protein of SEQ ID NO: 114.

16. The method according to claim 7 , wherein one or more of the mutations are effected by a mutagenic treatment of the potato plant.

17. The method according to claim 16 , wherein the mutagenic treatment is effected with a mutagen or with radiation.

18. An isolated potato plant produced from the method according to claim 7 , wherein the potato plant comprises the mutation in the first dmr6 gene of SEQ ID NO: 113 and has reduced expression of the first DMR6 protein of SEQ ID NO: 112 as compared to a corresponding wild-type potato plant.

19. A seed, tissue, or plant part of the potato plant according to claim 18 , wherein the seed, tissue, or plant part comprises the mutation in the first dmr6 gene of SEQ ID NO: 113 and has reduced expression of the first DMR6 protein of SEQ ID NO: 112.

20. An isolated potato plant produced from the method according to claim 7 , wherein the potato plant comprises the mutation in the second dmr6 gene of SEQ ID NO: 115 and has reduced expression of the second DMR6 protein of SEQ ID NO: 114 as compared to a corresponding wild-type potato plant.

21. A seed, tissue, or plant part of the potato plant according to claim 20 , wherein the seed, tissue, or plant part comprises the mutation in the second dmr6 gene of SEQ ID NO: 115 and has reduced expression of the second DMR6 protein of SEQ ID NO: 114.

22. An isolated potato plant produced from the method according to claim 7 , wherein the potato plant comprises the mutation in the first dmr6 gene of SEQ ID NO: 113 and has reduced expression of the first DMR6 protein of SEQ ID NO: 112 as compared to a corresponding wild-type potato plant, and further comprises the mutation in the second dmr6 gene of SEQ ID NO: 115 and has reduced expression of the second DMR6 protein of SEQ ID NO: 114 as compared to a corresponding wild-type potato plant.

23. A seed, tissue, or plant part of the potato plant according to claim 22 , wherein the potato plant comprises the mutation in the first dmr6 gene of SEQ ID NO: 113 and has reduced expression of the first DMR6 protein of SEQ ID NO: 112 as compared to a corresponding wild-type potato plant, and further comprises the mutation in the second dmr6 gene of SEQ ID NO: 115 and has reduced expression of the second DMR6 protein of SEQ ID NO: 114 as compared to a corresponding wild-type potato plant.

Assignees

• Enza Zaden Beheer Bv

Inventors

• Van Damme Mireille Maria Augusta
• Van Den Ackerveken Augustinus Franciscus Johannes Maria
• Van Schie Christianus Cornelis Nicolaas
• Zeilmaker Tieme

Classifications

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• C12N15/8239

  pathogen inducible · CPC title

• C12N15/8282

  for fungal resistance · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title