Disease resistant plants

Patent metadata

Field	Value
Publication number	US-9546373-B2
Application number	US-201414528707-A
Country	US
Kind code	B2
Filing date	Oct 30, 2014
Priority date	Feb 1, 2007
Publication date	Jan 17, 2017
Grant date	Jan 17, 2017

Abstract

The present invention relates to a plant, which is resistant to a pathogen of viral, bacterial, fungal or oomycete origin, wherein the plant has a reduced level, reduced activity or complete absence of DMR6 protein as compared to a plant that is not resistant to the said pathogen, in particular organisms of the Fungi or the phylum Oomycota. The invention further relates to a method for obtaining a plant, which is resistant to a pathogen of viral, bacterial, fungal or oomycete origin, comprising reducing the endogenous level or activity of DMR6 protein in the plant. In addition, the invention relates to the use of a DMR6 promotor for providing disease resistant plants.

First claim

The invention claimed is:

1. An isolated cucumber plant which is resistant to a pathogen, wherein the cucumber plant has a reduced level or reduced activity of DMR6 protein as compared to a cucumber plant that is not resistant to said pathogen, wherein said pathogen is Pseudoperonospora cubensis , said cucumber plant has a non-natural mutation introduced into the dmr6 gene of SEQ ID NO: 66 and said cucumber plant has a reduced level or reduced activity of the DMR6 protein of SEQ ID NO: 67.

2. The plant as claimed in claim 1 , wherein the mutation in the dmr6 gene leads to an amino acid substitution in the DMR6 protein.

3. A method for obtaining a cucumber plant which is resistant to a pathogen, the method comprising:
reducing an endogenous level of the DMR6 protein of SEQ ID NO: 67 in a cucumber plant by introducing a mutation into the dmr6 gene of SEQ ID NO: 66 to produce a cucumber plant that is resistant to Pseudoperonospora cubensis.

4. The method of claim 3 , wherein said reducing the endogenous level of the DMR6 protein in the cucumber plant is achieved by reducing expression of the dmr6 gene of SEQ ID NO: 66.

5. The method of claim 4 , wherein reducing expression of the dmr6 gene of SEQ ID NO: 66 is achieved by gene silencing or RNAi.

6. The method of claim 3 , wherein the mutation results in one or more amino acid changes that leads to a reduced enzymatic activity of the DMR6 protein of SEQ ID NO: 67.

7. The method of claim 3 , wherein the mutation is effected by a mutagenic treatment of the cucumber plant plant.

8. The method according to claim 7 , wherein the mutagenic treatment is effected with a mutagen or with radiation.

9. A seed, tissue, or plant part of the plant according to claim 1 , wherein the seed, tissue, or plant part comprises the mutation in the dmr6 gene of SEQ ID NO: 66 and has a reduced level or reduced activity of the DMR6 protein of SEQ ID NO: 67.

10. A plant produced by the method according to claim 3 , wherein the plant comprises the mutation in the dmr6 gene of SEQ ID NO. 66 and has a reduced level or reduced activity of the DMR6 protein of SEQ ID NO: 67.

11. A seed, tissue, or plant part of the plant according to claim 10 , wherein the seed, tissue, or plant part comprises the mutation in the dmr6 gene of SEQ ID NO: 66 and has a reduced level or reduced activity of the DMR6 protein of SEQ ID NO: 67.

Assignees

• Enza Zaden Beheer Bv

Inventors

• Van Damme Mireille Maria Augusta
• Van Den Ackerveken Augustinus Franciscus Johannes Maria

Classifications

• C12N15/8218 Primary

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• A01H1/06

  Processes for producing mutations, e.g. treatment with chemicals or with radiation (specific mutations prepared by genetic engineering on plant cell or plant tissues C12N15/00 {; process for producing transgenic plants C12N15/82}) · CPC title

• C12N15/8282

  for fungal resistance · CPC title

• C12Y114/11

  with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11) · CPC title

• C12N15/01

  Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor · CPC title