Phytophthora Resistant Plants Belonging to the Solanaceae Family

Patent metadata

Field	Value
Publication number	US-2016333370-A1
Application number	US-201415111285-A
Country	US
Kind code	A1
Filing date	Jan 14, 2014
Priority date	Jan 14, 2014
Publication date	Nov 17, 2016
Grant date	—

Abstract

Provided herein is a plant belonging to the Solanaceae family wherein said plant includes a genetic trait providing Phytophthora resistance and wherein said resistance trait is encoded by a combination of at least two genes having a reduced expression, or transcription, of said genes or a reduced activity of proteins encoded by said genes as compared to said plant belonging to Solanaceae family being susceptible to Phytophthora.

First claim

1 .- 11 . (canceled)

12 . An isolated potato plant which is resistant to Phytophthora infestans comprising a non-natural modification introduced into its genome that results in:
reduced expression or transcription of genes encoding proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2, or reduced activity of proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2, as compared to a potato plant that is not resistant to Phytophthora infestans.

13 . The potato plant as claimed in claim 12 , wherein the modification is gene silencing.

14 . The potato plant as claimed in claim 13 , wherein genes having the nucleotide sequences of SEQ ID NO: 7 and SEQ ID NO: 8 are silenced.

15 . The potato plant as claimed in claim 12 , wherein the modification results in reduced activity of proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2 as compared to a potato plant that is not resistant to Phytophthora infestans.

16 . The potato plant as claimed in claim 12 , wherein the modification results in reduced activity of proteins having the amino acid sequence of SEQ ID NO: 1 and SEQ ID NO: 2 as compared to a potato plant that is not resistant to Phytophthora infestans.

17 . A seed, tissue, or plant part of the potato plant according to claim 12 , wherein the seed, tissue, or plant part comprises the modification that results in:
reduced expression or transcription of genes encoding proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2, or reduced activity of proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2, as compared to a potato plant that is not resistant to Phytophthora infestans.

18 . A method for obtaining a potato plant which is resistant to Phytophthora infestans , the method comprising modifying the potato plant by:
reducing expression or transcription of genes encoding proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2, or reducing activity of proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2, as compared to a potato plant that is not resistant to Phytophthora infestans.

19 . The method of claim 18 , wherein the modification comprises reducing expression or transcription of genes encoding proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2 as compared to a potato plant that is not resistant to Phytophthora infestans , and wherein the modification is effected through gene silencing.

20 . The method of claim 19 , wherein genes having the nucleotide sequences of SEQ ID NO: 7 and SEQ ID NO: 8 are silenced.

21 . The method of claim 18 , wherein the modification comprises reducing activity of proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2 as compared to a potato plant that is not resistant to Phytophthora infestans.

22 . A potato plant produced from the method according to claim 18 , wherein the plant has:
reduced expression or transcription of genes encoding proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2, or reduced activity of proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2, as compared to a potato plant that is not resistant to Phytophthora infestans.

23 . A seed, tissue, or plant part of the potato plant according to claim 22 , wherein the seed, tissue, or plant part has:
reduced expression or transcription of genes encoding proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2, or reduced activity of proteins having at least 90% sequence identity to SEQ ID NO: 1 and SEQ ID NO: 2, as compared to a potato plant that is not resistant to Phytophthora infestans.

Assignees

• Enza Zaden Beheer Bv

Inventors

• Van Schie Christianus Cornelis Nicolaas
• Posthuma Karin Ingeborg
• Zeilmaker Tieme
• Kroon Maria Theresia
• De Boer Geert Johannes

Classifications

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• C12N15/8282

  for fungal resistance · CPC title

• C12N15/8279 Primary

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title