Use of M4 metalloprotease in wort production

Patent metadata

Field	Value
Publication number	US-10450539-B2
Application number	US-201615572872-A
Country	US
Kind code	B2
Filing date	Jun 3, 2016
Priority date	Jun 4, 2015
Publication date	Oct 22, 2019
Grant date	Oct 22, 2019

Abstract

A method of preparing a wort with an increased level of free amino nitrogen (FAN) comprising: a) preparing a mash from a grist comprising malt and/or adjunct; and b) adding a M4 mettalloprotease obtainable from Actinobacteria; and wherein the amount of free amino nitrogen (FAN) in the wort is increased as compared to a wort produced in the absence of the M4 metalloprotease.

First claim

The invention claimed is:

1. A method of preparing a wort with an increased level of free amino nitrogen (FAN) comprising:
a) preparing a mash from a grist comprising malt and/or adjunct; and
b) adding an M4 metalloprotease obtainable from Actinobacteria to the mash; and

wherein the amount of free amino nitrogen (FAN) in the wort is increased as compared to a wort produced in the absence of the M4 metalloprotease.

2. The method according to claim 1 , wherein the M4 metalloprotease has at least 80% sequence identity to the mature polypeptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, and SEQ ID NO:11.

3. The method according to claim 1 , wherein the M4 metalloprotease is a variant of the polypeptide of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:11 comprising a substitution, deletion, and/or insertion at one or more positions.

4. The method according to claim 1 , wherein the M4 metalloprotease consists of the polypeptide of SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:11.

5. The method according to claim 1 , wherein the M4 metalloprotease is added to the mash or to the wort.

6. The method according to claim 1 , further comprising adding an alpha amylase to the mash.

7. The method according to claim 1 , further comprising adding a beta glucanase to the mash.

8. The method according to claim 1 , further comprising adding a pullulanase to the mash.

9. The method according to claim 1 , further comprising adding a xylanase To the mash.

10. The method according to claim 1 , further comprising adding a lipase to the mash.

11. The method according to claim 1 , wherein the M4 metalloprotease is added in an amount of 1-100 mg enzyme protein per kg grist.

12. The method according to claim 1 , wherein the grist comprises at least 10% (w/w) adjunct.

13. The method according to claim 1 , wherein the adjunct is selected from the group consisting of barley, rice, corn, wheat, sorghum and cassava.

14. The method according to claim 1 , further comprising fermenting the wort to obtain a beer.

15. The method according to claim 1 , wherein the mashing is done in the absence of a protein rest.

16. The method according to claim 1 , wherein the amount of free amino nitrogen (FAN) is increased by at least 20% when the protease according to the invention is added in an amount of 10 mg Enzyme Protein per kg grist as compared to a wort produced in the absence of the M4 metalloprotease.

Assignees

• Novozymes As

Inventors

• Lunde Christina
• Gjermansen Morten

Classifications

• C12Y302/01

  Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1) · CPC title

• C12C7/04

  Preparation or treatment of the mash · CPC title

• C12Y302/01041

  Pullulanase (3.2.1.41) · CPC title

• C12C7/00

  Preparation of wort (malt extract C12C1/18) · CPC title

• C12C5/006

  Beta-glucanase or functionally equivalent enzymes · CPC title