Mesoporous catalysts of magnetic nanoparticles and free-radical-producing enzymes, and methods of use

Patent metadata

Field	Value
Publication number	US-10316313-B2
Application number	US-201615380104-A
Country	US
Kind code	B2
Filing date	Dec 15, 2016
Priority date	Mar 10, 2011
Publication date	Jun 11, 2019
Grant date	Jun 11, 2019

Abstract

A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.

First claim

What is claimed is:

1. A composition comprising self-assembled mesoporous aggregates of magnetic nanoparticles and a lactoperoxidase, wherein said lactoperoxidase is magnetically-entrapped without a bonding agent in mesopores of said self-assembled mesoporous aggregates of magnetic nanoparticles, and said lactoperoxidase functions by converting a diffusible substrate into a diffusible product.

2. The composition of claim 1 , wherein said self-assembled mesoporous aggregates of magnetic nanoparticles have an iron oxide composition.

3. The composition of claim 1 , wherein said self-assembled mesoporous aggregates of magnetic nanoparticles have a magnetic nanoparticle size distribution in which at least 90% of magnetic nanoparticles have a size of at least 3 nm and up to 30 nm, and an aggregated particle size distribution in which at least 90% of said self-assembled mesoporous aggregates of magnetic nanoparticles have a size of at least 10 nm and up to 500 nm.

4. The composition of claim 1 , wherein said self-assembled mesoporous aggregates of magnetic nanoparticles possess a saturated magnetization of at least 10 emu/g.

5. The composition of claim 1 , wherein said self-assembled mesoporous aggregates of magnetic nanoparticles possess a remanent magnetization up to 5 emu/g.

6. The composition of claim 1 , wherein said lactoperoxidase is contained in said self-assembled mesoporous aggregates of magnetic nanoparticles in up to 100% of saturation capacity.

7. The composition of claim 1 , wherein said mesopores are characterized by a pore size distribution in which at least 90% of said pore volume is attributed to pores having a pore size of at least 2 nm and up to 20 nm.

8. The composition of claim 1 , wherein said mesopores are characterized by a pore size distribution in which at least 95% of said pore volume is attributed to pores having a pore size of at least 2 nm and up to 20 nm.

9. The composition of claim 1 , further comprising an oxidase.

10. The composition of claim 9 , wherein said oxidase is glucose oxidase.

11. The composition of claim 1 , wherein said self-assembled mesoporous aggregates of magnetic nanoparticles and lactoperoxidase reside within submicrometric particles having a size of at least 20 nanometers.

12. A composition comprising self-assembled mesoporous aggregates of magnetic nanoparticles and an enzyme, wherein said enzyme is magnetically-entrapped without a bonding agent in mesopores of said self-assembled mesoporous aggregates of magnetic nanoparticles, and said enzyme functions by converting a diffusible substrate into a diffusible product, wherein said enzyme is selected from the group consisting of EC 1.1.2, EC 1.10.2, EC 1.14 oxidoreductase, cytochrome-b5 reductase, cytochrome-c reductase, and cytochrome-c peroxidase.

13. The composition of claim 12 , wherein said self-assembled mesoporous aggregates of magnetic nanoparticles have an iron oxide composition.

14. The composition of claim 12 , wherein said self-assembled mesoporous aggregates of magnetic nanoparticles have a magnetic nanoparticle size distribution in which at least 90% of magnetic nanoparticles have a size of at least 3 nm and up to 30 nm, and an aggregated particle size distribution in which at least 90% of said self-assembled mesoporous aggregates of magnetic nanoparticles have a size of at least 10 nm and up to 500 nm.

15. The composition of claim 12 , wherein said self-assembled mesoporous aggregates of magnetic nanoparticles possess a saturated magnetization of at least 10 emu/g.

16. The composition of claim 12 , wherein said self-assembled mesoporous aggregates of magnetic nanoparticles possess a remnant magnetization up to 5 emu/g.

17. The composition of claim 12 , wherein said enzyme is contained in said self-assembled mesoporous aggregates of magnetic nanoparticles in up to 100% of saturation capacity.

18. The composition of claim 12 , wherein said mesopores are characterized by a pore size distribution in which at least 95% of said pore volume is attributed to pores having a pore size of at least 2 nm and up to 20 nm.

19. The composition of claim 12 , wherein said mesopores are characterized by a pore size distribution in which at least 90% of said pore volume is attributed to pores having a pore size of at least 2 nm and up to 20 nm.

Assignees

• Univ Cornell

Inventors

• Corgie Stephane C
• Kahawong Patarawan
• Giannelis Emmanuel P
• Walker Larry P

Classifications

• C12Y111/01007

  Peroxidase (1.11.1.7), i.e. horseradish-peroxidase · CPC title

• C12Y110/02001

  L-Ascorbate--cytochrome-b5 reductase (1.10.2.1) · CPC title

• C12P2201/00

  Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis · CPC title

• C02F2305/08

  Nanoparticles or nanotubes · CPC title

• B82Y25/00

  Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance · CPC title