Mesoporous catalysts of magnetic nanoparticles and free-radical-producing enzymes, and methods of use

Patent metadata

Field	Value
Publication number	US-9597672-B2
Application number	US-201214004311-A
Country	US
Kind code	B2
Filing date	Mar 9, 2012
Priority date	Mar 10, 2011
Publication date	Mar 21, 2017
Grant date	Mar 21, 2017

Abstract

A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.

First claim

What is claimed is:

1. A composition comprising self-assembled mesoporous aggregates of magnetic nanoparticles and a first free-radical producing enzyme, wherein said first enzyme is magnetically-entrapped without a bonding agent in mesopores formed by said aggregates of magnetic nanoparticles and said first enzyme functions by converting a diffusible substrate into a diffusible product.

2. The composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles have an iron oxide composition.

3. The composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles have a magnetic nanoparticle size distribution in which at least 90% of magnetic nanoparticles have a size of at least 3 nm and up to 30 nm, and an aggregated particle size distribution in which at least 90% of said mesoporous aggregates of magnetic nanoparticles have a size of at least 10 nm and up to 500 nm.

4. The composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles possess a saturated magnetization of at least 10 emu/g.

5. The composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles possess a remanent magnetization up to 5 emu/g.

6. The composition of claim 1 , wherein said free-radical-producing enzyme is contained in said mesoporous aggregates of magnetic nanoparticles in up to 100% of saturation capacity.

7. The composition of claim 1 , wherein said first free-radical-producing enzyme is an EC 1.11 oxidoreductase.

8. The composition of claim 7 , wherein said free-radical-producing enzyme is comprised of a peroxidase.

9. The composition of claim 8 , wherein said peroxidase is a class III peroxidase.

10. The composition of claim 9 , wherein said class III peroxidase is horseradish peroxidase.

11. The composition of claim 1 , wherein said mesopores are characterized by a pore size distribution in which at least 90% of the pore volume is attributed to pores having a pore size of at least 2 nm and up to 20 nm.

12. The composition of claim 1 , further comprising a second enzyme that is an EC 1.1.3 enzyme, and wherein said first enzyme is a peroxidase.

13. The composition of claim 12 , wherein said EC 1.1.3 enzyme is glucose oxidase EC 1.1.3.4.

14. The composition of claim 12 , wherein said peroxidase is horseradish peroxidase.

15. The composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme further comprise a metallic surface layer.

16. Microparticles comprising the composition of claim 1 .

17. The microparticles of claim 16 , wherein said microparticles comprise magnetite.

18. The microparticles of claim 16 , wherein said microparticles are ferromagnetic submicrometric particles having a size of at least 20 nanometers.

19. The composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles have a magnetic nanoparticle size distribution in which at least 50% of magnetic nanoparticles have a size of at least 1 nm and up to 50 nm, and an aggregated particle size distribution in which at least 90% of said mesoporous aggregates of magnetic nanoparticles have a size of at least 10 nm and up to 500 nm, and wherein said free-radical producing enzyme is an oxidoreductase belonging to the EC1 family of enzymes.

Assignees

• Corgie Stephane C
• Kahawong Patarawan
• Giannelis Emmanuel P
• Walker Larry P
• Univ Cornell

Inventors

• Corgie Stephane C
• Kahawong Patarawan
• Giannelis Emmanuel P
• Walker Larry P

Classifications

• C02F3/342

  characterised by the enzymes used · CPC title

• C12N9/0004

  Oxidoreductases (1.) · CPC title

• C12Y111/01007

  Peroxidase (1.11.1.7), i.e. horseradish-peroxidase · CPC title

• C12Y110/02001

  L-Ascorbate--cytochrome-b5 reductase (1.10.2.1) · CPC title

• C12N11/14 Primary

  Enzymes or microbial cells immobilised on or in an inorganic carrier · CPC title