Molecular characterization device

Patent metadata

Field	Value
Publication number	US-10228348-B2
Application number	US-201615004248-A
Country	US
Kind code	B2
Filing date	Jan 22, 2016
Priority date	Apr 6, 2005
Publication date	Mar 12, 2019
Grant date	Mar 12, 2019

Abstract

Provided is a solid state support structure including an aperture having a molecular entrance and a molecular exit. A first reservoir is in fluidic communication with the molecular entrance of the aperture and contains a molecule-bearing liquid solution. A second reservoir is in fluidic communication with the molecular exit of the aperture for containing a molecule-bearing liquid solution. A first liquid channel is connected to the first reservoir within less than about 300 microns of the aperture in the support structure and includes molecule-bearing liquid solution for delivery to the first reservoir. A second liquid channel is connected to the second reservoir for accepting molecule-bearing liquid solution from the second reservoir. An electrical connection between the first reservoir and the second reservoir imposes an electrical bias between the first reservoir and the second reservoir for driving the molecule-bearing liquid solution through the aperture in the solid state support structure.

First claim

We claim:

1. A molecular characterization device comprising:
a solid state support structure including an aperture having a molecular entrance and a molecular exit;
a first reservoir in fluidic communication with the molecular entrance of the aperture and containing a molecule-bearing liquid solution;
a second reservoir in fluidic communication with the molecular exit of the aperture for containing a molecule-bearing liquid solution;
a first liquid channel connected to the first reservoir within less than about 300 microns of the aperture in the solid state support structure, the first liquid channel including the molecule-bearing liquid solution for delivery to the first reservoir;
a second liquid channel connected to the second reservoir for accepting the molecule-bearing liquid solution from the second reservoir; and
an electrical connection between the first reservoir and the second reservoir that imposes an electrical bias between the first reservoir and the second reservoir for driving the molecule-bearing liquid solution through the aperture in the solid state support structure.

2. The molecular characterization device of claim 1 wherein the first liquid channel and the second liquid channel each having a diameter less than about 200 microns.

3. The molecular characterization device of claim 1 further comprising:
a first tube connected to the first liquid channel for supplying the molecule-bearing liquid solution to the first liquid channel;
a second tube connected to the second liquid channel for accepting the molecule-bearing liquid solution from the second liquid channel; and
wherein the first tube and the second tube each have a diameter less than about 200 microns.

4. The molecular characterization device of claim 1 wherein the molecular entrance of fluidic communication between the first reservoir and the solid state support structure is less than 1000 μm 2 in area.

5. The molecular characterization device of claim 1 wherein the molecule to be characterized comprises a biomolecule.

6. The molecular characterization device of claim 1 wherein the molecule comprises a polymer molecule.

7. The molecular characterization device of claim 1 wherein the molecule comprises a biopolymer molecule.

8. The molecular characterization device of claim 7 wherein the molecule is selected from the group consisting of proteins, polynucleic acids, DNA, and RNA.

9. The molecular characterization device of claim 1 wherein the aperture comprises a pore, extending through the thickness of the support structure, and having a diameter permitting only a single molecule at a time to move through the pore.

10. The molecular characterization device of claim 1 wherein the aperture has a diameter less than about 100 nm.

11. The molecular characterization device of claim 1 wherein the aperture has a diameter less than about 10 nm.

12. The molecular characterization device of claim 1 wherein the aperture is coated with an electrically insulating material layer.

13. The molecular characterization device of claim 1 wherein the support structure comprises an electrically insulating membrane in which the aperture is provided.

14. The molecular characterization device of claim 1 wherein the aperture has a length, through a thickness of the solid state support structure between the molecular entrance and the molecular exit, that is between about 0.1 nm and about 800 nm.

Assignees

• Harvard College

Inventors

• Branton Daniel
• Golovchenko Jene A

Classifications

• C12Q1/6869 Primary

  Methods for sequencing · CPC title

• C12Q2565/631

  being a biochannel or pore · CPC title

• G01N33/48721

  Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68) · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• G01N27/44743

  Introducing samples · CPC title