Botanical extracts and compounds from Castanea plants and methods of use

Patent metadata

Field	Value
Publication number	US-10195241-B2
Application number	US-201615192514-A
Country	US
Kind code	B2
Filing date	Jun 24, 2016
Priority date	Jun 26, 2015
Publication date	Feb 5, 2019
Grant date	Feb 5, 2019

Abstract

This disclosure relates to extracts from chestnut plants and compositions comprising compounds contained therein. In certain embodiments, the extracts are derived from the leaves of a Castanea plant. In certain embodiments, the disclosure relates to methods of treating or preventing bacterial infections, acne, and other related uses.

First claim

The invention claimed is:

1. A wound dressing or wound rinse comprising compounds in an extract, wherein the wound dress comprises an absorbent pad and optionally an adhesive, and wherein the extract comprises a leaf derived mixture of compounds from a Castanea plant wherein the extracting process comprises the steps of:
mixing a leaf with methanol under conditions such that leaf compounds dissolves in the methanol and removing the methanol providing a methanol derived mixture of compounds,
partitioning the methanol derived mixture of compounds in hexane and water providing a water derived mixture of compounds,
partitioning the water derived mixture of compounds by mixing the water with ethyl acetate under conditions such that leaf compounds dissolve in the ethyl acetate and removing the ethyl acetate providing an ethyl acetate derived mixture of compounds; and
purifying the ethyl acetate derived mixture of compounds by liquid chromatography through silica with a mobile phase comprising hexane and ethyl acetate;
wherein the mobile phase comprises increasing amounts of ethyl acetate, and a mobile phase fraction is isolated comprising a leaf derived mixture of compounds which does not contain chlorogenic acid, ellagic acid, hyperoside, isoquercitrin or rutin.

2. The extract of claim 1 , wherein the mixture of compounds comprises components having the following formulas and relative abundance,
C 39 H 59 O 8 or C 38 H 55 O 9 , or mixture, with a relative abundance of 0.1 to 0.9%;
C 35 H 59 O 6 with a relative abundance of 0.1 to 0.9%;
C 31 H 49 O 6 with a relative abundance of 1.0 to 2.0%;
C 32 H 51 O 7 with a relative abundance of 0.1 to 0.9%;
C 30 H 48 O 5 with a relative abundance of 3.0 to 5.0%;
C 30 H 48 O 6 with a relative abundance of 6.0 to 8.0%; and
C 30 H 45 O 5 with a relative abundance of 2.0 to 4.0%.

3. The extract of claim 2 , further comprises additional components of different compounds having the following formula and relative abundance,
C 35 H 59 O 6 with a relative abundance of 0.2 to 0.4;
C 27 H 23 O 18 is with a relative abundance of 0.05 to 0.30;
C 39 H 31 O 15 with a relative abundance of 0.50 to 0.80;
C 17 H 11 O 8 or C 20 H 11 O 4 N 2 , or mixture, with a relative abundance of 0.5 to 1.0; and
C 34 H 29 O 15 with a relative abundance of 0.2 to 0.4%.

Assignees

• Univ Emory
• Univ Iowa Res Found

Inventors

• Quave Cassandra L
• Lyles James
• Horswill Alexander R

Classifications

• A61K45/06

  Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca · CPC title

• A61K36/49 Primary

  Fagaceae (Beech family), e.g. oak or chestnut · CPC title

• A61Q19/10

  Washing or bathing preparations · CPC title

• A61L15/44

  Medicaments · CPC title

• A61K2236/15

  involving mechanical treatment, e.g. chopping up, cutting or grinding · CPC title