Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells

Patent metadata

Field	Value
Publication number	US-10188082-B2
Application number	US-201715655663-A
Country	US
Kind code	B2
Filing date	Jul 20, 2017
Priority date	Jun 6, 2012
Publication date	Jan 29, 2019
Grant date	Jan 29, 2019

Abstract

The invention relates to methods of increasing the genetic progress of a line, breed or herd of swine through the use of sex-selected sperm cells in artificial insemination techniques. The invention also encompasses methods of artificially inseminating a swine via deep intrauterine catheter or via a laparoscopic procedure, which allow the use of reduced doses of sex-selected sperm cells.

First claim

What is claimed is:

1. A method of producing an embryo or zygote in a genetic nucleus comprising the steps of:
collecting a semen sample from a boar from the genetic nucleus;
sorting the semen sample into at least two subpopulations of sperm cells, wherein at least 60% of a first subpopulation bears X-chromosomes or Y-chromosomes;
fertilizing an egg from a sow from the genetic nucleus with one or more sperm cells from the first subpopulation to produce a zygote or embryo;
genotyping the zygote or embryo to obtain a genotype;
determining an estimated breeding value for the zygote or embryo; and
selecting the zygote or embryo to develop into offspring that is used for breeding in the genetic nucleus based on the estimated breeding value.

2. The method of claim 1 , wherein the step of fertilizing an egg comprises in vitro fertilization.

3. The method of claim 1 , further comprising the step of transferring the zygote or embryo to a recipient sow.

4. The method of claim 3 , wherein said recipient sow is a sow in the genetic nucleus.

5. The method of claim 3 , wherein said recipient sow is a sow in a second genetic nucleus.

6. The method of claim 3 , wherein said recipient sow is a sow in a daughter nucleus herd, multiplier herd or commercial farm.

Assignees

• Inguran Llc

Inventors

• Moreno Juan
• Bevier Gregg
• Dobrinsky John

Classifications

• A61B10/0012

  Ovulation-period determination (for animals A61D17/002) · CPC title

• G01N15/00

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• A61B2034/2057

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• A01K67/027

  New or modified breeds of vertebrates · CPC title

• A61B34/20

  Surgical navigation systems; Devices for tracking or guiding surgical instruments, e.g. for frameless stereotaxis · CPC title