Sialic acid-specific binding affinity lectin from the mushroom Hericium erinaceum

Patent metadata

Field	Value
Publication number	US-10150799-B2
Application number	US-201615158407-A
Country	US
Kind code	B2
Filing date	May 18, 2016
Priority date	Nov 18, 2013
Publication date	Dec 11, 2018
Grant date	Dec 11, 2018

Abstract

The present invention concerns lectins isolated from the fruiting body of a novel Hericium erinaceum (deposit number: KCTC 12499BP) NEU-1L strain which bind specifically to sialic acid. The invention further pertains to uses of such lectinsn abd to processes for their preparation thereby. The lectin of the present invention can be useful as an active ingredient of a composition or a kit for measuring or detecting glycoproteins, glycopeptides, glycolipids, sugar precursors or oligosaccharides containing sialic acid moieties, or further for measuring or detecting cell lines, bacteria and viruses containing sialoglycoconjugates.

First claim

What is claimed is:

1. A method for measurement or quantification of a sialylated conjugate composed of glycoproteins, glycopeptides, glycolipids, sugar precursors, or oligosaccharides having sialic acid moieties, the method comprising the following steps:
a) contacting a test sample comprising the sialylated conjugate with a lectin which is or can be obtained from a Hericium erinaceum NEU-1L strain deposited under the deposit number KCTC 12499BP, wherein the lectin is obtained by
(i) preparing a crude extract from the fruiting body of the Hericium erinaceum NEU-1L strain; and
(ii) purifying the lectin from the crude extract by using a resin immobilized with one or more of glycoproteins, glycopeptides, glycolipids, oligosaccharides, or monosaccharides,

wherein the lectin comprises the protein N-terminal sequence of SEQ ID NO: 7 or NO: 8, and has a molecular weight of 15 kDa˜20 kDa, or wherein the lectin comprises the protein N-terminal sequence of SEQ ID NO: 9, and has a molecular weight of 50 kDa˜75 kDa.; and
b) measuring or quantifying the glycoproteins, glycopeptides, glycolipids, sugar precursors, or oligosaccharides binding to the lectin.

2. The method of claim 1 , wherein the test sample comprises cells, bacteria, or viruses comprising the sialylated conjugate on their surface, and wherein the method further comprises:
c) measuring or quantifying the cells, bacteria, or viruses binding to the lectin.

3. The method of claim 1 , wherein the lectin is provided in a kit.

4. The method of claim 2 , wherein the lectin is provided in a kit.

5. The method of claim 1 , wherein the lectin binds specifically to one or more of the following glycan structures:
sialylated glycan wherein NeuAc or NeuGc(α2→3) is added to a Gal or Glc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→6) is added to a Gal or Glc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→3) is added to a Gal(β1→4)Glc or Glc(β1→4)Glc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→6) is added to a Gal(β1→4)Glc or Glc(β1→4)Glc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→3) is added to a Gal(β1→4)GlcNAc or Gal(β1→3)GlcNAc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→6) is added to a Gal(β1→4)GlcNAc or Gal(β1→3)GlcNAc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→3) is added to a Gal(β1→3)[αFuc(1→4)]GlcNAc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→6) is added to a Gal(β1→3)[αFuc(1→4)]GlcNAc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→3) is added to a Gal(β1→4)[αFuc(1→3)]GlcNAc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→6) is added to a Gal(β1→4)[αFuc(1→3)]GlcNAc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→8) is added to a NeuAc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→8) is added to a NeuAc(α2→3) Gal(β1→4)GlcNAc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→8) is added to a NeuAc(α2→8)NeuAc(α2→3) Gal(β1→4)GlcNAc residue;
sialylated glycan wherein NeuAc or NeuGc(α2→8) is added to a NeuAc(α2→8) [NeuAc(α2→8)]nNeuAc(α2→3) Gal(β1→4)GlcNAc residue; and
sialylated glycan wherein NeuAc or NeuGc(α2→8) is added to a [NeuAc]n residue.

Assignees

• Korea Res Inst Bioscience & Biotechnology

Inventors

• Kim Seounghun

Classifications

• G01N33/582

  with fluorescent label · CPC title

• C07K14/375 Primary

  from Basidiomycetes · CPC title

• G01N2333/42

  Lectins, e.g. concanavalin, phytohaemagglutinin · CPC title

• G01N33/6848

  Methods of protein analysis involving mass spectrometry · CPC title

• C07K14/37 Primary

  from fungi · CPC title