Histamine releaser contained in human sweat

Patent metadata

Field	Value
Publication number	US-9709576-B2
Application number	US-201615248106-A
Country	US
Kind code	B2
Filing date	Aug 26, 2016
Priority date	Jun 28, 2012
Publication date	Jul 18, 2017
Grant date	Jul 18, 2017

Abstract

Provided are a sweat allergy antigen, an antibody capable of binding to the antigen specifically, and others, which are produced utilizing a microorganism-originated protein that exists in sweat allergy patient in a dissolved state or a partial peptide of the protein.

First claim

The invention claimed is:

1. A method of detecting antibody binding to a sweat allergy antigen protein, comprising
contacting a biological sample from a human with a sweat allergy antigen protein selected from the group consisting of
(i) a protein comprising an amino acid sequence having 90% or more sequence identity to the amino acid sequence of SEQ ID NO: 1; and
(ii) a peptide comprising an amino acid sequence having 90% or more sequence identity to the amino acid sequence of any of SEQ ID NO: 2 to 7; and
detecting the antibody binding to the sweat allergy antigen protein.

2. The method of claim 1 , further comprising measuring an amount of the antibody binding to a sweat allergy antigen protein.

3. The method of claim 1 , further comprising determining an amount of the antibody binding to a sweat allergy antigen protein in the sample based on results from detecting the antibody that binds to the protein or peptide, wherein the contacting the sample with the sweat allergy antigen protein is performed using the sweat allergy antigen protein at a plurality of known concentrations.

4. The method of claim 1 , wherein the sweat allergy antigen protein is a protein comprising the amino acid sequence of SEQ ID NO: 1.

5. The method of claim 1 , wherein the sweat allergy antigen protein is a protein consisting of the amino acid sequence of SEQ ID NO: 1.

6. The method of claim 1 , wherein the sweat allergy antigen protein is a peptide comprising the amino acid sequence of any of SEQ ID NO: 2 to 7.

7. The method of claim 1 , wherein the sweat allergy antigen protein is a peptide consisting of the amino acid sequence of any of SEQ ID NO: 2 to 7.

8. The method of claim 1 , wherein the biological sample from a human includes sweat, skin washings, a solution of an extract of skin, a serum, or a plasma.

9. The method of claim 1 , wherein the detecting the antibody is performed using Western blotting or enzyme-linked immunosorbent assay (ELISA).

10. The method of claim 1 , wherein the sweat allergy antigen protein is a protein secreted from a fungus body of Malassezia globosa , has a molecular weight of about 17 kDa, and binds to (i) serum derived from a sweat allergy patient and/or (ii) smith2 antibody.

11. The method of claim 1 , wherein the sweat allergy antigen protein is a protein present in a fungus body of Malassezia globosa , has a molecular weight of about 30 kDa, and binds to (i) serum derived from a sweat allergy patient and/or (ii) smith2 antibody.

12. A method of diagnosing a sweat allergy or a disease relating to a sweat allergy antigen protein, comprising
detecting antibody binding to a sweat allergy antigen protein in a sample from a human according to the method of claim 1 .

Assignees

• Univ Hiroshima

Inventors

• Hide Michihiro
• Hiragun Takaaki
• Ishii Kaori
• Mihara Shoji
• Hiragun Makiko
• Schroeder Jens-M

Classifications

• A61P37/08

  Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14) · CPC title

• A61P17/00

  Drugs for dermatological disorders · CPC title

• C07K2317/76

  Antagonist effect on antigen, e.g. neutralization or inhibition of binding · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• G01N2800/24

  Immunology or allergic disorders · CPC title