Use of galerina marginata genes and proteins for peptide production

Patent metadata

Field	Value
Publication number	US-9932571-B2
Application number	US-201715437359-A
Country	US
Kind code	B2
Filing date	Feb 20, 2017
Priority date	Nov 9, 2007
Publication date	Apr 3, 2018
Grant date	Apr 3, 2018

Abstract

The present invention relates to compositions and methods comprising genes and peptides associated with cyclic peptides and cyclic peptide production in mushrooms. In particular, the present invention relates to using genes and proteins from Galerina species encoding peptides specifically relating to amatoxins in addition to proteins involved with processing cyclic peptide toxins. In a preferred embodiment, the present invention also relates to methods for making small peptides and small cyclic peptides including peptides similar to amanitin. Further, the present inventions relate to providing kits for making small peptides.

First claim

The invention claimed is:

1. A cell comprising a recombinant prepropeptide nucleic acid encoding a proline-containing peptide operably linked to a promoter, and a heterologous fungal prolyl oligopeptidase nucleic acid encoding an amino acid sequence selected from the group consisting of SEQ ID NO: 236, 237, 348, 716, and 722.

2. The cell of claim 1 , which is a prokaryotic cell.

3. The cell of claim 1 , which is a eukaryotic cell.

4. The cell of claim 1 , which is an insect cell.

5. The cell of claim 1 , which is a mammalian cell.

6. A bacterial, fungal, or algal organism comprising a cell comprising a recombinant prepropeptide nucleic acid encoding a proline-containing peptide operably linked to a promoter, and a heterologous fungal prolyl oligopeptidase nucleic acid encoding an amino acid sequence selected from the group consisting of SEQ ID NO: 236, 237, 348, 716, and 722.

7. The organism of claim 6 , which is a eukaryotic organism.

8. The organism of claim 6 , which is a fungus.

9. A method of making a synthetic cyclized peptide, comprising:
a) providing,
i) a cell comprising a fungal prolyl oligopeptidase with an amino acid sequence selected from the group consisting of SEQ ID NOs: 236, 237, 348, 716, and 722;
ii) a recombinant prepropeptide nucleic acid comprising a nucleic acid sequence encoding a proline-containing prepropeptide, and
iii) amino acids for making the synthetic cyclized peptide; and
b) growing the cell under conditions for expressing said prepropeptide and thereby making the synthetic cyclized peptide.

10. The method of claim 9 , wherein the amino acids for making the synthetic cyclized peptide comprise D-amino acids.

11. The method of claim 9 , wherein the peptide is at least six amino acids in length.

12. The method of claim 9 , wherein the peptide is up to fifteen amino acids in length.

13. The method of claim 9 , wherein the peptide is a bicyclic peptide.

14. A method of making a peptide from a recombinant prepropeptide sequence, comprising:
(a) providing a fungal prolyl oligopeptidase comprising an amino acid sequence selected from SEQ ID NO: 236, 237, 348, 716, and 722, and a proline-containing prepropeptide; and
(b) amino acids for making the synthetic cyclized peptide comprising D-amino acids; and
(c) contacting the fungal prolyl oligopeptidase with the prepropeptide and the amino acids to make the peptide.

15. The method of claim 14 , wherein the peptide is at least six amino acids in length.

16. The method of claim 14 , wherein the peptide is up to fifteen amino acids in length.

17. The method of claim 14 , wherein the peptide is a bicyclic peptide.

18. A composition comprising a proline-containing prepropeptide, amino acids for making a synthetic cyclized peptide comprising D-amino acids, and a fungal prolyl oligopeptidase comprising an amino acid sequence with in a sequence selected from amino acid SEQ ID NO: 236, 237, 348, 716, and 722, or optionally a fungal prolyl oligopeptidase comprising an amino acid sequence with a sequence selected from amino acid SEQ ID NO: 236, 237, 348, 716, and 722 with one amino acid change.

Assignees

• Univ Michigan State

Inventors

• Hallen-Adams Heather E
• Scott-Craig John S
• Walton Jonathan D
• Luo Hong

Classifications

• C12N9/58 Primary

  derived from fungi · CPC title

• C12P21/06

  produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00) · CPC title

• C07K7/64

  Cyclic peptides containing only normal peptide links · CPC title

• C07K14/375

  from Basidiomycetes · CPC title

• C12Y304/21026

  Prolyl oligopeptidase (3.4.21.26), i.e. proline-specific endopeptidase · CPC title