Methods and compositions for determining bovine ovulation rate

Patent metadata

Field	Value
Publication number	US-10098332-B2
Application number	US-201414321036-A
Country	US
Kind code	B2
Filing date	Jul 1, 2014
Priority date	Jul 1, 2013
Publication date	Oct 16, 2018
Grant date	Oct 16, 2018

Abstract

Described herein are methods and compositions for modulating bovine birth rate by following a breeding scheme based on the presence of the trio haplotype, which is strongly linked to the propensity to give birth to multiple calves in one event.

First claim

What is claimed is:

1. A method for increasing beef cattle production, comprising:
(a) obtaining a biological sample from a bull or cow to be bred;
(b) genotyping a bull or cow for the presence or absence of a trio haplotype (trio + ) using a panel of genetic markers consisting of:
(i) a first polymorphism comprising a 4 base pair insertion or deletion at base pair 13,683,941 of bovine chromosome 10 (BTA10);
(ii) a second polymorphism comprising a C to A transversion at base pair 14,263,362 of BTA10; and
(iii) a third polymorphism comprising an A to G transition at base pair 14,270,483 of BTA10;

wherein the presence of the first, second and third polymorphisms identifies the bull or cow as trio + ; and
(c) selectively breeding the bull or cow identified as trio + ;
wherein a bred trio + cow has an increased likelihood, relative, to a cow of unknown trio + haplotype status, of giving birth to multiple calves in one pregnancy: and a trio + female progeny of a bred trio + bull or bred trio + cow has an increased likelihood, relative to a cow of unknown trio haplotype status, of giving birth to multiple calves in one pregnancy, whereby multiple calve births provide an increase in beef cattle production.

2. The method of claim 1 , wherein the biological sample comprises an autologous genomic DNA sample of the bull or cow to be bred.

3. The method of claim 1 , wherein the breeding comprises an artificial insemination step.

4. The method of claim 1 , wherein the first, second and third polymorphisms are identified by genotyping assays independently-selected from: Polymerase Chain Reaction Amplification of a Specific Allele (PASA); and Restriction Fragment Length Polymorphism Analysis of a Polymerase Chain Reaction-Amplified Fragment (PCR-RFLP).

5. The method of claim 4 , wherein: (a) the first polymorphism is identified by PASA assay using a primer set comprising the oligonucleotides set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, and SEQ ID NO:4, or PCR-RFLP assay using a primer set comprising the oligonucleotides set forth in SEQ ID NO:5 and 6; (b) the second polymorphism is identified by PCR-RFLP assay using a primer set comprising the oligonucleotides set forth in SEQ ID NO:7 and 8; and (c) the third polymorphism is identified b PCR-RFLP assay using a primer set comprising the oligonucleotides set forth in SEQ ID NO: 9 and 10.

Assignees

• Wisconsin Alumni Res Found

Inventors

• Kirkpatrick Brian Wayne

Classifications

• C12Q1/6883

  for diseases caused by alterations of genetic material · CPC title

• C12Q2600/124

  Animal traits, i.e. production traits, including athletic performance or the like · CPC title

• A01K67/02 Primary

  Breeding vertebrates · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title