Synthetic bi-directional plant promoter

Patent metadata

Field	Value
Publication number	US-9988638-B2
Application number	US-201514938643-A
Country	US
Kind code	B2
Filing date	Nov 11, 2015
Priority date	Nov 11, 2014
Publication date	Jun 5, 2018
Grant date	Jun 5, 2018

Abstract

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a Arabidopsis thaliana Ubiquitin-10 gene promoter or Cassava Vein Mosaic Virus promoter, and the full-length nucleotide sequence elements from a Cassava Vein Mosaic Virus promoter. Some embodiments relate to a synthetic CsVMV bi-directional promoter that functions in plants to promote transcription of two operably linked nucleotide sequences.

First claim

What is claimed is:

1. A nucleic acid molecule comprising a bidirectional promoter selected from the group consisting of SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, and SEQ ID NO:13.

2. The nucleic acid molecule of claim 1 , wherein the bidirectional promoter is SEQ ID NO:10.

3. The nucleic acid molecule of claim 1 , wherein the bidirectional promoter is SEQ ID NO:11.

4. The nucleic acid molecule of claim 1 , wherein the bidirectional promoter is SEQ ID NO:12.

5. The nucleic acid molecule of claim 1 , wherein the bidirectional promoter is SEQ ID NO:13.

6. The nucleic acid molecule of claim 1 , further comprising a first polynucleotide encoding a polypeptide of interest operably linked to the 3′ end of the bidirectional promoter.

7. The nucleic acid molecule of claim 6 , further comprising a second polynucleotide encoding a polypeptide of interest operably linked to the 5′ end of the bidirectional promoter.

8. The nucleic acid molecule of claim 6 , wherein the polypeptide of interest is involved in a plant trait.

9. The nucleic acid molecule of claim 8 , wherein the plant trait is selected from the group consisting of insect resistance, herbicide tolerance, nitrogen use efficiency, water use efficiency, and nutritional quality.

10. A method for producing a transgenic plant cell, the method comprising:
transforming a plant cell with the nucleic acid molecule of claim 1 , wherein the nucleic acid molecule further comprises at least one polynucleotide encoding a polypeptide of interest operably linked to the bidirectional promoter, thereby producing a transgenic plant cell.

11. The method according to claim 10 , wherein the plant cell is comprised in a plant, plant part, plant cell culture, or plant tissue culture.

12. The method according to claim 10 , wherein the plant cell is transformed by a transformation method selected from the group consisting of Agrobacterium -mediated transformation, biolistics transformation, silicon carbide transformation, protoplast transformation, and liposome transformation.

13. The method according to claim 10 , the method further comprising:
regenerating a transgenic plant from the transgenic plant cell.

14. The method according to claim 10 , wherein the plant cell is selected from the group consisting of Arabidopsis , tobacco, soybean, canola, cotton, maize, rice, Brachypodium , and wheat.

15. A transgenic plant cell comprising an expression cassette that comprises a bidirectional promoter selected from the group consisting of SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, and SEQ ID NO:13 operably linked to a polynucleotide encoding a polypeptide of interest.

16. The transgenic plant cell of claim 15 , wherein the expression cassette is stably integrated in the genome of the cell.

17. The transgenic plant cell of claim 16 , wherein the polypeptide of interest is involved in an agronomic trait.

18. The transgenic plant cell of claim 17 , wherein the agronomic trait is selected from the group consisting of insect resistance, herbicide tolerance, nitrogen use efficiency, water use efficiency, and nutritional quality.

19. The transgenic plant cell of claim 17 , wherein the agronomic trait is herbicide tolerance.

20. The transgenic plant cell of claim 19 , wherein the polynucleotide comprises aad-1.

21. A transgenic plant commodity product comprising the nucleic acid molecule of claim 1 .

22. The transgenic plant commodity product of claim 21 , wherein the commodity product is selected from the group consisting of grain, meal, flour, oil, or fiber.

23. The transgenic plant cell of claim 15 , wherein the transgenic plant cell is from a plant selected from the group consisting of Arabidopsis , tobacco, soybean, canola, cotton, maize, rice, Brachypodium , and wheat.

24. The transgenic plant cell of claim 23 , wherein the transgenic plant cell is a soybean cell.

25. The nucleic acid molecule of claim 1 , further comprising a first polynucleotide encoding a polypeptide of interest operably linked to the 5′ end of the bidirectional promoter.

26. The nucleic acid molecule of claim 25 , wherein the polypeptide of interest is involved in a plant trait.

27. The nucleic acid molecule of claim 26 , wherein the plant trait is selected from the group consisting of insect resistance, herbicide tolerance, nitrogen use efficiency, water use efficiency, and nutritional quality.

28. The transgenic plant cell of claim 15 , wherein the polynucleotide encoding the polypeptide of interest is constitutively expressed.

29. The transgenic plant cell of claim 15 , further comprising a second polynucleotide encoding a polypeptide of interest operably linked to the other end of the bidirectional promoter from the first polynucleotide.

30. The transgenic plant cell of claim 29 , wherein the transgenic plant cell is from a plant selected from the group consisting of Arabidopsis , tobacco, soybean, canola, cotton, maize, rice, Brachypodium , and wheat.

31. A transgenic plant or seed comprising an expression cassette that comprises a bidirectional promoter selected from the group consisting of SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, and SEQ ID NO:13 operably linked to a polynucleotide encoding a polypeptide of interest.

32. The transgenic plant or seed of claim 31 , wherein the polypeptide of interest is involved in an agronomic trait.

33. The transgenic plant or seed of claim 32 , wherein the agronomic trait is selected from the group consisting of insect resistance, herbicide tolerance, nitrogen use efficiency, water use efficiency, and nutritional quality.

34. The transgenic plant or seed of claim 31 , wherein the plant or seed is a monocotyledonous plant or seed.

35. The transgenic plant or seed of claim 31 , wherein the plant or seed is a dicotyledonous plant or seed.

36. The transgenic plant or seed of claim 31 , wherein the transgenic plant or seed is selected from the group consisting of Arabidopsis , tobacco, soybean, canola, cotton, maize, rice, Brachypodium , and wheat.

37. The transgenic plant or seed of claim 31 , further comprising a second polynucleotide encoding a polypeptide of interest operably linked to the other end of the bidirectional promoter from the first polynucleotide.

Assignees

• Dow Agrosciences Llc

Inventors

• Kumar Sandeep
• Cicak Toby
• Robinson Heather Leigh
• Pareddy Dayakar Reddy
• Chen Wei

Classifications

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8274

  for herbicide resistance · CPC title

• C12Y207/07047

  Streptomycin 3''-adenylyltransferase (2.7.7.47) · CPC title

• C12N9/1241

  Nucleotidyltransferases (2.7.7) · CPC title