Recombinant DNA constructs and methods for controlling gene expression

US9212370B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-9212370-B2
Application numberUS-72667310-A
CountryUS
Kind codeB2
Filing dateMar 18, 2010
Priority dateDec 21, 2004
Publication dateDec 15, 2015
Grant dateDec 15, 2015

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Abstract

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The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

First claim

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What is claimed is: 1. A recombinant DNA construct comprising a promoter functional in a plant cell and operably linked to DNA that transcribes to RNA comprising: (a) at least one exogenous miRNA recognition site recognizable by a mature miRNA, wherein said mature miRNA is encoded by a DNA sequence selected from SEQ ID NOs:36, 40, 41, or 42 and is expressed in a specific cell of a plant, and (b) target RNA to be suppressed in said specific cell, wherein said target RNA is to b…

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What does patent US9212370B2 cover?
The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppressi…
Who is the assignee on this patent?
Allen Edwards M, Gilbertson Larry A, Heisel Sara E, and 4 more
What technology area does this patent fall under?
Primary CPC classification C12N15/8218. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Dec 15 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).