Method of producing sugar liquid

Patent metadata

Field	Value
Publication number	US-9976160-B2
Application number	US-201414903642-A
Country	US
Kind code	B2
Filing date	Jul 8, 2014
Priority date	Jul 9, 2013
Publication date	May 22, 2018
Grant date	May 22, 2018

Abstract

A method of producing a sugar liquid from biomass includes decomposing a fermentation inhibitor contained in a sugar aqueous solution obtained from biomass with a microorganism incapable of utilizing glucose and/or xylose or a crude enzyme derived from the microorganism, wherein the fermentation inhibitor includes one or more compounds selected from the group consisting of courmaric acid, coumaramide, ferulic acid, ferulamide, vanillin, vanillic acid, acetovanillone, furfural, and 3-hydroxymethylfurfural.

First claim

The invention claimed is:

1. A method of producing a sugar liquid from biomass, comprising:
(a) decomposing one or more fermentation inhibitor(s) contained in a sugar aqueous solution obtained from biomass with a microorganism incapable of utilizing glucose and/or xylose or a crude enzyme extract obtained from the microorganism, to obtain a sugar liquid having a reduced fermentation inhibitory activity compared to the sugar aqueous solution; and
(b) subjecting the sugar liquid obtained in step (a) to membrane concentration and/or evaporation concentration to increase sugar concentration,
wherein the one or more fermentation inhibitor(s) are selected from the group consisting of coumaric acid, coumaramide, ferulic acid, ferulamide, vanillin, vanillic acid, acetovanillone, furfural, and 3-hydroxymethylfurfural, and
the microorganism incapable of utilizing glucose and/or xylose is a Delftia microorganism ( Delftia sp.).

2. The method according to claim 1 , wherein the microorganism incapable of utilizing glucose and/or xylose is selected from the group consisting of Delftia acidovorans, Delftia lacustris, Delftia tsuruhatensis , and Delftia litopenaei.

3. The method according to claim 1 , wherein the sugar aqueous solution is a sugar aqueous solution obtained by hydrolyzing cellulose-containing biomass.

4. The method according to claim 3 , further comprising preparing the sugar aqueous solution by subjecting the cellulose-containing biomass to one or more treatments selected from the group consisting of an acid treatment, an alkali treatment, a hydrothermal treatment, and an enzyme treatment.

5. The method according to claim 1 , wherein the sugar aqueous solution comprises blackstrap molasses.

6. The method according to claim 1 , wherein the fermentation inhibitor decomposition treatment is at a monosaccharide concentration of 0.1 g/L to 100 g/L.

7. The method according to claim 1 , wherein the fermentation inhibitor decomposition treatment is at a pH of 6 to 11.

Assignees

• Toray Industries

Inventors

• Kurihara Hiroyuki
• Ishizuka Hiroko
• Yamada Katsushige

Classifications

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C13K1/04

  Purifying · CPC title

• C13K13/00

  Sugars not otherwise provided for in this class · CPC title

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C13B50/00

  Sugar products, e.g. powdered, lump or liquid sugar; Working-up of sugar (C13B40/00, C13B45/00 take precedence; confectionery A23G3/00) · CPC title