Particle control device

Patent metadata

Field	Value
Publication number	US-9965867-B2
Application number	US-201415031802-A
Country	US
Kind code	B2
Filing date	Oct 2, 2014
Priority date	Nov 1, 2013
Publication date	May 8, 2018
Grant date	May 8, 2018

Abstract

A micro object control apparatus for controlling motion of a micro object within a medium includes a light source, an optical vortex generation unit, an objective lens, an imaging unit, an analysis unit, and a movement unit. The analysis unit acquires first motion information of the micro object based on the image data in which the micro object optically trapped with the optical vortex is imaged by setting the focal position of the optical vortex to a first position, acquires second motion information of the micro object based on the image data in which the micro object optically trapped with the optical vortex is imaged by setting the focal position of the optical vortex to a second position, and evaluates a state of an optical trap of the micro object with the optical vortex by comparing the first motion information and the second motion information.

First claim

The invention claimed is:

1. A micro object control apparatus for controlling motion of a micro object in a medium, the apparatus comprising:
a light source configured to output light;
an optical vortex generator configured to input the output light and output an optical vortex;
an objective lens configured to focus the output vortex and irradiate the micro object in the medium with the focused optical vortex to optically trap the micro object;
a camera configured to capture an image of the micro object through the objective lens and output image data;
an analyzer connected to the camera and configured to analyze the motion of the micro object based on the image data output from the camera; and
a movement unit configured to move a focal position of the focused optical vortex by the objective lens in an optical axis direction of the objective lens, wherein
the analyzer
acquires first motion information of the micro object based on the image data output from the camera in which the micro object optically trapped with the optical vortex is imaged by setting the focal position of the optical vortex by the objective lens to a first position by the movement unit,
acquires second motion information of the micro object based on the image data output from the camera in which the micro object optically trapped with the optical vortex is imaged by setting the focal position of the optical vortex by the objective lens to a second position different from the first position with respect to the optical axis direction by the movement unit, and
evaluates a state of an optical trap of the micro object with the optical vortex by comparing the first motion information and the second motion information.

2. The micro object control apparatus according to claim 1 , wherein the optical vortex generator is a spatial light modulator having a plurality of pixels two-dimensionally arrayed, modulating an amplitude and a phase of light in each pixel, and outputting the light.

3. The micro object control apparatus according to claim 1 , wherein the analyzer analyzes a circular motion or an elliptic motion of the micro object based on the image data output from the camera.

4. The micro object control apparatus according to claim 1 , wherein the analyzer acquires, as each of the first motion information and the second motion information, at least any one of a velocity, an angular velocity, an acceleration, a revolution radius, and a physical quantity calculated from these of the micro object.

5. The micro object control apparatus according to claim 1 , wherein the analyzer evaluates, in comparing the first motion information and the second motion information, whether or not a difference between the first motion information and the second motion information is a predetermined value or less.

6. The micro object control apparatus according to claim 1 , wherein the movement unit includes a piezoelectric element moving the focal position of the optical vortex.

Assignees

• Hamamatsu Photonics Kk

Inventors

• OTSU Tomoko
• Ando Taro
• Itoh Hiroyasu
• Toyoda Haruyoshi
• Ohtake Yoshiyuki
• Takiguchi Yuu

Classifications

• G21K1/30

  for confining neutral particles or handling confined neutral particles, e.g. atom traps · CPC title

• H04N23/67

  Focus control based on electronic image sensor signals · CPC title

• G06T7/248 Primary

  involving reference images or patches · CPC title

• G06T2207/30241

  Trajectory · CPC title

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title