Making c4+ products in bacteria
US-2015376658-A1 · Dec 31, 2015 · US
Alkane oxidation by modified hydroxylases
US9963720B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9963720-B2 |
| Application number | US-201615224900-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 1, 2016 |
| Priority date | Mar 28, 2005 |
| Publication date | May 8, 2018 |
| Grant date | May 8, 2018 |
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- Title
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- Abstract
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Abstract
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This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.
First claim
Opening claim text (preview).
What is claimed is: 1. An isolated or recombinant polypeptide comprising residues 1 to 455 of the amino acid sequence of SEQ ID NO:125 with up to 25 conservative amino acid substitutions excluding residues 47, 52, 74, 78, 82, 94, 142, 175, 184, 188, 205, 226, 236, 252, 255, 290, 328, 353, 366, 464, 443 and 710, wherein the polypeptide catalyzes the conversion of a (C 1 -C 12 )alkane to an alcohol. 2. The polypeptide of claim 1 , comprising up to 10 amino acid substitutions. 3. The polypeptide of claim 1 , comprising up to 5 amino acid substitutions. 4. The polypeptide of claim 1 , wherein the alkane is selected from the group consisting of methane (CH 4 ), ethane (C 2 H 6 ), propane (C 3 H 8 ), butane (C 4 H 10 ), pentane (C 5 H 12 ), hexane (C 6 H 14 ), heptane (C 7 H 16 ), octane (C 8 H 18 ), nonane (C 9 H 20 ), decane (C 10 H 22 ), undecane (C 11 H 24 ), and dodecane (C 12 H 26 ). 5. The polypeptide of claim 1 , wherein the alcohol is selected from the group consisting of methanol, ethanol, propanol, butanol, pentanol, hexanol, heptanol, octanol, nonanol, decanol, undecanol, and dodecanol. 6. A method of converting a C1 to C12 alkane to an alcohol comprising contacting the C1 to C12 alkane with a polypeptide of claim 1 under conditions to produce the alcohol. 7. The method of claim 6 , wherein the contacting is in a cell-free system.
Assignees
Inventors
Classifications
acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title
Unspecific monooxygenase (1.14.14.1) · CPC title
- C12P7/04Primary
acyclic · CPC title
NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase · CPC title
NADPH-cytochrome P450 reductase (1.6.2.4) · CPC title
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- What does patent US9963720B2 cover?
- This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.
- Who is the assignee on this patent?
- The California Institute Of Tech, California Inst Of Techn
- What technology area does this patent fall under?
- Primary CPC classification C12P7/04. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue May 08 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).