Making c4+ products in bacteria
US-2015376658-A1 · Dec 31, 2015 · US
US9150889B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9150889-B2 |
| Application number | US-201113522288-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 15, 2011 |
| Priority date | Jan 15, 2010 |
| Publication date | Oct 6, 2015 |
| Grant date | Oct 6, 2015 |
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The disclosure provides a process that converts CO 2 to higher alcohols (e.g. isobutanol) using electricity as the energy source. This process stores electricity (e.g. from solar energy, nuclear energy, and the like) in liquid fuels that can be used as high octane number gasoline substitutes. Instead of deriving reducing power from photosynthesis, this process derives reducing power from electrically generated mediators, either H 2 or formate. H 2 can be derived from electrolysis of water. Formate can be generated by electrochemical reduction of CO 2 . After delivering the reducing power in the cell, formate becomes CO 2 and recycles back. Therefore, the biological CO 2 fixation process can occur in the dark.
Opening claim text (preview).
What is claimed is: 1. A recombinant microorganism capable of using H 2 or formate for reduction of CO 2 and wherein the microorganism produces an alcohol selected from the group consisting of 1-propanol, isobutanol, 1-butanol, 2-methyl 1-butanol, 3-methyl 1-butanol and 2-phenylethanol from CO 2 as the carbon source, wherein the alcohol is produced from a metabolite comprising a 2-keto acid and wherein the microorganism comprises expression of a heterologous or overexpression of…
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