Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors
US-2015368676-A1 · Dec 24, 2015 · US
US9963684B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9963684-B2 |
| Application number | US-201415125785-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 29, 2014 |
| Priority date | Mar 13, 2014 |
| Publication date | May 8, 2018 |
| Grant date | May 8, 2018 |
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This invention relates to a novel formaldehyde dehydrogenase expressed by a formaldehyde dehydrogenase gene and having independent reduction activity for formic acid, a method of preparing the formaldehyde dehydrogenase from a strain transformed with a recombinant expression vector including the gene, and a method of producing formaldehyde from formic acid through a reduction reaction of the formaldehyde dehydrogenase.
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The invention claimed is: 1. A method of producing formaldehyde from a substrate through a reduction reaction with formaldehyde dehydrogenase having reduction activity and comprising an amino acid sequence of SEQ ID NO:2 by treating the substrate with the formaldehyde dehydrogenase using NADH as a coenzyme, wherein the substrate is formic acid.
Formaldehyde dehydrogenase (1.2.1.46) · CPC title
containing a carbonyl group · CPC title
Genes encoding for enzymes or proenzymes · CPC title
acting on the aldehyde or oxo group of donors (1.2) · CPC title
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