Process for the extraction of polyhydroxyalkanoates from biomass
US-2015368393-A1 · Dec 24, 2015 · US
US9169501B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9169501-B2 |
| Application number | US-201414301882-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 11, 2014 |
| Priority date | May 11, 2012 |
| Publication date | Oct 27, 2015 |
| Grant date | Oct 27, 2015 |
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Disclosed is a method for producing biodegradable polymer and ethanol converted from carbon source by using recombinant microorganisms, comprising the steps of: (A) providing recombinant microorganisms transformed with plasmids containing at least a gene encoding for glycerol utilizing enzyme and a gene encoding for polyhydroxyalkanoate synthase; (B) culturing the recombinant microorganisms in a medium containing glycerol; (C) inducing expression of the genes of step (A), thereby obtaining polyhydroxyalkanoate and ethanol; and (D) recovering the polyhydroxyalkanoate and the ethanol; wherein the recombinant microorganisms have a glycerol utilization rate more than 90% (w/w), and have polyhydroxyalkanoate accumulated therein to a biomass content thereof at least 30% (w/w).
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What is claimed is: 1. A method for producing biodegradable polymer and ethanol converted from carbon source, comprising the steps of: (A) providing E. coli DSM 27723, said E. coli DSM 27723 containing plasmids encoding aldehyde reductase (alrd), aldehyde dehydroxygenase (aldH), and PhaCAB polyhydroxyalkanoate synthase; (B) culturing the E. coli DSM 27723 in a medium containing glycerol; (C) inducing expression of the E. coli DSM 27723 plasmid encoded aldehyde reducta…
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