Biological sensing method for separating biomolecule

Patent metadata

Field	Value
Publication number	US-9958453-B2
Application number	US-201615229111-A
Country	US
Kind code	B2
Filing date	Aug 5, 2016
Priority date	Mar 10, 2015
Publication date	May 1, 2018
Grant date	May 1, 2018

Abstract

A biological sensing device and a method for separating a biomolecule are provided. The biological sensing device includes an amino acid sequence and a signal-generating unit. The amino acid sequence includes SEQ ID NO: 1 or SEQ ID NO: 2, and is for binding with UlaG protein labeled on a biomolecule. The signal-generating unit connects to the amino acid sequence.

First claim

What is claimed is:

1. A method for separating a biomolecule, comprising:
providing a sample, the sample comprising a biomolecule having L-ascorbate-6-phosphate lactonase;
contacting the sample with an amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2;
binding the amino acid sequence with the L-ascorbate-6-phosphate lactonase, wherein SEQ ID NO: 1 or SEQ ID NO: 2 has physical contact with the L-ascorbate-6-phosphate lactonase; and
removing a portion of the sample not bound with the amino acid sequence to separate the biomolecule from the sample.

2. The method of claim 1 , further comprising modifying the L-ascorbate-6-phosphate lactonase onto the biomolecule.

3. The method of claim 1 , further comprising modifying the sample onto a substrate.

4. The method of claim 1 , further comprising connecting the amino acid sequence to a signal-generating unit.

Assignees

• Univ National Chiao Tung

Inventors

• Li Yaw-Kuen
• Chang Chia-Yu
• LI Bor-Ran

Classifications

• G01N33/56944 Primary

  Streptococcus · CPC title

• G01N33/6878

  in epitope analysis · CPC title

• G01N27/4145

  specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title

• G01N2333/3156

  from Streptococcus pneumoniae [Pneumococcus] · CPC title

• G01N1/405

  by adsorption or absorption · CPC title