Biological sensing device and method for separating biomolecule

Patent metadata

Field	Value
Publication number	US-2016266145-A1
Application number	US-201514740269-A
Country	US
Kind code	A1
Filing date	Jun 16, 2015
Priority date	Mar 10, 2015
Publication date	Sep 15, 2016
Grant date	—

Abstract

A biological sensing device and a method for separating a biomolecule are provided. The biological sensing device includes an amino acid sequence and a signal-generating unit. The amino acid sequence includes SEQ ID NO: 1 or SEQ ID NO: 2, and is for binding with UlaG protein labeled on a biomolecule. The signal-generating unit connects to the amino acid sequence.

First claim

What is claimed is:

1 . A biological sensing device, comprising:
an amino acid sequence, comprising SEQ ID NO: 1 or SEQ ID NO: 2, and being for binding with UlaG protein labeled on a biomolecule; and a signal-generating unit, connecting to the amino acid sequence.

2 . The biological sensing device of claim 1 , wherein the biomolecule is a protein, a cell, a bacterium, or a combination thereof.

3 . The biological sensing device of claim 2 , wherein the bacterium is a Gram-positive bacterium.

4 . The biological sensing device of claim 3 , wherein the Gram-positive bacterium is from the genus Streptococcus.

5 . The biological sensing device of claim 4 , wherein the Gram-positive bacterium from the genus Streptococcus is Streptococcus pneumoniae.

6 . The biological sensing device of claim 1 , wherein the signal-generating unit is a transistor, a fluorescent molecule, or a chip.

7 . A method for separating a biomolecule, comprising:
providing a sample, the sample comprising a biomolecule having UlaG protein; contacting the sample with an amino acid sequence comprising SEQ ID NO: 1 or SEQ ID NO: 2; binding the amino acid sequence with the UlaG protein; and removing a portion of the sample not bound with the amino acid sequence to separate the biomolecule from the sample.

8 . The method of claim 7 , further comprising modifying the UlaG protein onto the biomolecule.

9 . The method of claim 7 , further comprising modifying the sample onto a substrate.

10 . The method of claim 7 , further comprising connecting the amino acid sequence to a signal-generating unit.

Assignees

• Univ Nat Chiao Tung

Inventors

• Li Yaw-Kuen
• Chang Chia-Yu
• LI Bor-Ran

Classifications

• G01N2333/3156

  from Streptococcus pneumoniae [Pneumococcus] · CPC title

• G01N33/6878 Primary

  in epitope analysis · CPC title

• G01N33/68 Primary

  involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)} · CPC title

• G01N27/4145

  specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title

• G01N33/56944 Primary

  Streptococcus · CPC title