Method for using hexenol glycosyl transferase

Patent metadata

Field	Value
Publication number	US-9957539-B2
Application number	US-201414908889-A
Country	US
Kind code	B2
Filing date	Aug 1, 2014
Priority date	Aug 2, 2013
Publication date	May 1, 2018
Grant date	May 1, 2018

Abstract

A method for producing a hexenol glycoside using a hexenol glycosyltransferase. A transformant transformed with a gene encoding a hexenol glycosyltransferase and a method for preparing such a transformant.

First claim

The invention claimed is:

1. A method for producing a hexenol glycoside, comprising:
contacting an isolated, purified or extracted protein with a UDP-sugar and a hexenol molecule to cause glycosylation of the hexenol molecule and to thereby produce a hexenol glycoside;
wherein the isolated, purified or extracted protein has glycosylation activity on the hexenol molecule and is selected from the group consisting of:
(a) a protein consisting of the amino acid sequence of SEQ ID NO: 2 or 4;
(b) a protein consisting of the amino acid sequence of SEQ ID NO: 2 or 4, except that no more than 1 to 24 amino acids are deleted, substituted, inserted, and/or added; and
(c) a protein comprising an amino acid sequence that has 95% or more sequence identity with the amino acid sequence of SEQ ID NO: 2 or 4.

2. The method according to claim 1 , wherein the UDP-sugar is a UDP-hexose.

3. The method according to claim 2 , wherein the hexose of the UDP-hexose is selected from the group consisting of glucose, mannose and galactose.

4. The method according to claim 1 , further comprising purifying the hexenol glycoside.

5. A method for producing a hexenol glycoside, comprising:
providing a cell comprising a polynucleotide encoding a protein, wherein said cell expresses said protein, and said cell is a microorganism or a transgenic plant cell, and
contacting within or outside of the cell said protein with a UDP-sugar and a hexenol molecule to cause glycosylation of the hexenol molecule and to thereby produce a hexenol glycoside,
wherein said polynucleotide comprises a nucleotide sequence encoding said protein and an expression control region that controls expression of said nucleotide sequence and wherein the nucleotide sequence encoding said protein and/or the expression control region is heterologous to said cell,
wherein said protein has glycosylation activity on the hexenol molecule and is selected from the group consisting of:
(a) a protein consisting of the amino acid sequence of SEQ ID NO: 2 or 4;
(b) a protein consisting of the amino acid sequence of SEQ ID NO: 2 or 4, except that no more than 1 to 24 amino acids are deleted, substituted, inserted, and/or added; and
(c) a protein comprising an amino acid sequence that has 95% or more sequence identity with the amino acid sequence of SEQ ID NO: 2 or 4.

6. The method according to claim 5 , wherein the UDP-sugar is a UDP-hexose.

7. The method according to claim 6 , wherein the hexose of the UDP-hexose is selected from the group consisting of glucose, mannose and galactose.

8. The method according to claim 5 , further comprising purifying the hexenol glycoside.

9. The method according to claim 5 , wherein the transgenic plant cell is from a transformed whole plant, a transformed plant organ, a transformed plant tissue, transformed cultured plant cells, or from a progeny plant of a transgenic whole plant whose genome comprises the polynucleotide encoding the protein having glycosylation activity on the hexenol molecule.

Assignees

• Suntory Holdings Ltd

Inventors

• Ono Eiichiro

Classifications

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12Y204/00

  Glycosyltransferases (2.4) · CPC title

• C12N9/1048

  Glycosyltransferases (2.4) · CPC title

• C12P19/18 Primary

  produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins · CPC title