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Compositions and methods for the transfer of a hexosamine to a modified nucleotide in a nucleic acid
US9200260B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9200260-B2 |
| Application number | US-201313804804-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 14, 2013 |
| Priority date | Mar 15, 2012 |
| Publication date | Dec 1, 2015 |
| Grant date | Dec 1, 2015 |
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Abstract
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Nucleic acids comprising β-glucosaminyloxy-5-methylcytosine; compositions, kits and methods of producing the nucleic acids using a glycosyltransferase; and methods of using the nucleic acids are described.
First claim
Opening claim text (preview).
What is claimed is: 1. A cell free composition comprising: an isolated DNA β-glucosyltransferase; UDP-glucosamine (UDP-GlcN); and a restriction enzyme capable of cleaving a nucleic acid at a site comprising β-glucosyl-5-hydroxymethylcytosine but not a site comprising β-2-glucosaminyl-5-hydroxymethylcytosine. 2. The composition of claim 1 , further comprising a buffer having a pH between 6 and 8. 3. The composition of claim 2 , wherei…
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Chemistry & Metallurgy · mapped topic
- C12N9/0069Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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Frequently asked questions
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- What does patent US9200260B2 cover?
- Nucleic acids comprising β-glucosaminyloxy-5-methylcytosine; compositions, kits and methods of producing the nucleic acids using a glycosyltransferase; and methods of using the nucleic acids are described.
- Who is the assignee on this patent?
- New England Biolabs Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/0069. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 01 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).