Fermentation product containing equol-producing microorganism having maintained equol-producing ability, and method for producing same

Patent metadata

Field	Value
Publication number	US-9951360-B2
Application number	US-201514755550-A
Country	US
Kind code	B2
Filing date	Jun 30, 2015
Priority date	Sep 19, 2008
Publication date	Apr 24, 2018
Grant date	Apr 24, 2018

Abstract

An object of the present invention is to provide a fermented product containing equol-producing microorganisms in the state of living cells by which equol production ability is maintained.
When producing a fermented material by using an equol-producing microorganism, with soybean powder or soybean milk as raw materials, (1) preparing a mother starter by fermentation under anaerobic conditions by using an equol-producing microorganism in the presence of a daidzein species at pH 5.0 or higher, (2) preparing a bulk starter by fermentation under anaerobic conditions by using said mother starter in the presence of a daidzein species at pH 5.0 or higher, and (3) preparing a fermented material by fermentation by using said bulk starter in a medium containing soybean powder or soybean milk, enables production of a fermented material containing microorganisms in the state of living cells in which the equol production ability is maintained.

First claim

The invention claimed is:

1. A process for producing a fermented material comprising the steps of:
(1) conducting anaerobic fermentation in a first medium containing at least one type of daidzein species selected from the group consisting of daidzein glycoside, daidzein and dihydrodaidzein in an amount of 0.002 to 0.04 wt %, by using an equol-producing microorganism, in a state where a pH value of 5.0 to 8.0 is maintained to prepare a mother starter, wherein said first medium contains a carbohydrate at a concentration of 0.4 wt % or less;
(2) conducting fermentation by using the mother starter obtained in step (1) in a second medium containing the daidzein species in a state where a pH value of 5.0 to 8.0 is maintained to prepare a bulk starter, wherein said second medium contains a carbohydrate at a concentration of 0.4 wt % or less; and
(3) conducting fermentation by using the bulk starter obtained in step (2) in a medium containing soybean powder and/or soybean milk to obtain a fermented material.

2. The production process according to claim 1 , wherein the equol-producing microorganism is a lactic acid bacterium.

3. The production process according to claim 1 , wherein the equol-producing microorganism is Lactococcus garvieae.

4. A process for producing a fermented material comprising the steps of:
(I) conducting anaerobic fermenting in a first medium containing at least one type of daidzein species selected from the group consisting of daidzein glycoside, daidzein and dihydrodaidzein in an amount of 0.002 to 0.04 wt %, by using an equol-producing microorganism to prepare a mother starter, wherein said first medium contains a carbohydrate at a concentration of 0.4 wt % or less; and
(II) conducting fermentation by using the mother starter obtained in step (I) in a second medium containing the daidzein species and milk, in a state where a pH value of 4.6 to 7.0 is maintained to obtain a fermented material, wherein said second medium contains a carbohydrate at a concentration of 0.4 wt % or less,
wherein the medium used in steps (I) and (II) contains yeast extract, whey hydrolysate, casein hydrolysate, and yeast hydrolysate at a concentration of 0.1 wt % or more.

5. The production process according to claim 4 , wherein soybean hypocotyl extract is used as daidzein species.

6. The production process according to claim 4 , wherein the equol-producing microorganism is a lactic acid bacterium.

7. The production process according to claim 4 , wherein the equol-producing microorganism is Lactococcus garvieae.

Assignees

• Otsuka Pharma Co Ltd

Inventors

• Isono Yoshikazu
• Mori Hisako
• Ueno Tomomi
• Endo Rieko
• Kumemura Megumi
• Abiru Yasuhiro
• Uchiyama Shigeto

Classifications

• A61P3/06

  Antihyperlipidemics · CPC title

• A61P25/00

  Drugs for disorders of the nervous system · CPC title

• A61P19/10

  for osteoporosis · CPC title

• C07D311/58

  other than with oxygen or sulphur atoms in position 2 or 4 · CPC title

• A23L33/135

  Bacteria or derivatives thereof, e.g. probiotics · CPC title