Systems and methods for measuring cell signaling protein activity
US-2024230643-A9 · Jul 11, 2024 · US
US9181539B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9181539-B2 |
| Application number | US-201113101462-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 5, 2011 |
| Priority date | May 7, 2010 |
| Publication date | Nov 10, 2015 |
| Grant date | Nov 10, 2015 |
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The invention relates to the production of flavonoids and flavonoid precursors in cells through recombinant expression of tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI).
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We claim: 1. An isolated cell that recombinantly expresses genes encoding tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI), and wherein the cell produces one or more flavonoids or naringenin from glucose without additional precursor supplementation, and wherein the expression of the genes encoding TAL, 4CL, CHS and CHI is balanced in the cell by constitutive expression of CHS and CHI. 2. The isolat…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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